电生理方法集萃

General Methods Overview

·Electrophysiology Overview

·Clinical Electrophysiology – The Electroretinogram ERG by Donnell Creel (University of Utah)

·Electrophysiological Recordings (N. Wanaverbecq, Eccles Corner Neuroscience)

·Electrophysiological Techniques - Visually Evoked Cortical Potentials (VECP) and ElectroRetinoGram (ERG)

·Electrophysiological recordings in oocytes (P. Imbrici, Eccles Corner Neuroscience)

·The Nerve Impulse (Dr. Bezanilla, UCLA)

·Laboratory Experiments: Nerve Action Potential Simulation

Buffers and Solutions

·Solutions for slice preparation, slice maintenance and patch clamp recording (A. Semyanov, N. Wanaverbecq, R. Scott, Eccles Corner Neuroscience)

·A technique of fast drug application to outside-out patches (A. Semyanov, D.M. Kullmann, Eccles Corner Neuroscience)

Brain Slice Preparation

·in vitroSlice Preparation (Medical College of Georgia)

·Brain Slice Electrophysiology Methods (Winder Lab, Vanderbilt Medical Center)

·Preparation of acutely dissociated hippocampal neuron (A. Semyanov & N. Wanaverbecq, Eccles Corner Neuroscience)

Extracellular Recording

·Recording Single-Neuron Electrical Activity in the Human Brain (Matthew A. Howard III, M.D., University of Iowa)

·Extracellular Electrophysiology (Sean M. Montgomery, Rutgers University)

·Extracellular Recording from the Stomatogastric Ganglion of the Crab, Carcinus Maenus: Effects of Dopamine and Serotonin (Sean Montgomery & Errol Ruckert, Rutgers University)

·A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide (Craig S. Nunemaker, et al. University of Virginia)

·Extracellular single and multiple unit recording with carbon fibre microelectrodes

Intracellular Recording

·Intracellular recording and staining inLymnaea

·Intracellular Features Predicted by ExtracellularRecordingsin the Hippocampus in Vivo

Multiple Neuronal Recording

·Large-scale recording of neuronal ensembles (Gy?rgy Buzsáki, Nature Neuroscience)

·Using multi-neuron population recordings for neural prosthetics (John K Chapin, Nature Neuroscience)

·Multiple neural spike train data analysis: state-of-the-art and future challenges (Emery N Brown, et al. Nature Neuroscience)

Patch Clamp

·Methods for Study of Ion Channels

·Electrophysiology - The Patch Clamp Technique (Assmann Lab, Pennsylvania State University

·Patch Clamp Technique (direct electrical measurement of ion channel currents while simultaneously controlling the cell’s membrane potential)

·Patch Clamp Technique - Introducing patch clamp technique through flash movie

·Whole Cell Patch Clamp Technique (Albert Einstein College of Medicine)

·High-resolution scanning patch-clamp: new insights into cell function (The FASEB Journal)

·Patch Clamp Measurements Applied to Neurons

·Ion-selective microelectrode, sharp electrode, whole-cell clamp and gramicidin perforated-patch recordings (K. Lamsa, Eccles Corner Neuroscience)

·Combined patch clamp recording and mRNA expression profiling in individual neurons (Douglas Coulter's Lab, The Children's Hospital of Philadelphia)

·A Novel Method for Characterizing Synaptic Noise in Cortical Neurons (Alain Destexhe, et al. Neurocomputing)

·High-resolution scanning patch-clamp: new insights into cell function (The FASEB Journal)

Microdialysis

·The Microdialysis Technique – The Principle (Microdialysis.se)

·Introduction to Microdialysis Technique (University of Groningen)

·Microdialysis - Neurotransmitter Measurement (Emory University)

·Simultaneous Determination of Caffeine From Blood, Brain and Muscle Using Microdialysis in an Awake Rat and Effect of Caffeine on Rat Activity

·Neurochemical: microdialysis and postmortem measures of dopamine and norepinephrine release using HPLC-EC

·A microdialysis method for the recovery of IL-1beta, IL-6 and nerve growth factor from human brain in vivo

·Microdialysis methods for measuring human metabolism

·Microdialysis techniques in the study of brain and skeletal muscle

·Microsensor and microdialysis technology. Advanced techniques in the management of severe head injury