Sample : 5 µL
(Left) 1 ng/mL each (in H2O)
(Right) 1 ng/mL each (in 35 mg/mL BSA solution)
Column : Shodex ODP2 HP-2B (2.0 mm I.D. x 50 mm) Eluent : (A); 0.05 % NH3 aq. (pH11.0) (B); CH3CN Linear gradient; (B %) 5 % (0 min), 5 % to 90 % (5 to 10 min), 5 % (10.1 to 15 min) Flow rate : 0.2 mL/min Detector : UV (210 nm), ESI-MS/MS (MRM positive) Column temp. : 30 ℃