Sample : Molecular weight marker
1. Glutamate dehydrogenase
2. Lactate dehydrogenase
3. Enolase
4. Adenylate kinase
5. Cytochrome c
Columns : Shodex PROTEIN KW-802.5, KW-803 (8.0 mm I.D. x 300 mm each) Eluent : 50 mM Phosphate buffer (pH7.0) + 0.3 M NaCl Flow rate : 1.0 mL/min Detector : UV (280 nm) Column temp. : Room temp.