供应盐酸克仑特罗酶联免疫(ELISA)

盐酸克仑特罗酶联免疫 (ELISA) 快速检测试剂盒

1 概要

盐酸克仑特罗（俗称瘦肉精）为一种人工合成的 β 兴奋剂，具有扩张支气管的作用，常用来防治哮喘、肺气肿等肺部疾病。当其应用剂量达到治疗量的 5~10 倍时，具有能量重分配作用，可使肌肉合成增加，脂肪沉积减少，因此俗称为 “ 瘦肉精 ” 。在畜牧业生产中一些非法使用者将其作为一种生长促进剂添加到动物饲料中，用以改善胴体的品质，同时也造成食品中盐酸克仑特罗（瘦肉精）的残留。当人们食用盐酸克仑特罗残留的产品后会出现一系列的中毒症状。我国禁止在畜牧业中使用盐酸克仑特罗。

本试剂盒可用于定量、定性检测饲料、动物尿液以及动物组织等样本中盐酸克仑特罗的残留。

2 原理

本试剂盒采用直接竞争 ELISA 法检测样本中的盐酸克仑特罗。在微孔板上预包被抗盐酸克仑特罗单克隆抗体，样本或标准溶液中的盐酸克仑特罗和 HRP 标记的盐酸克仑特罗竞争结合微孔板中抗盐酸克仑特罗单克隆抗体，洗涤后用 H₂O₂-TMB 底物系统显色，样本吸光度值与其所含盐酸克仑特罗量成负相关，与标准曲线比较即可得出盐酸克仑特罗含量。

3 试剂盒组份

每个试剂盒中的试剂可进行 96 个测量（包括标准测定），试剂盒组份：

包被有抗盐酸 克仑特罗抗体 微孔板 ····················································· 96 孔

盐酸克仑特罗标准溶液 ································································· 1.5 mL / 瓶（ 6 瓶）

分别为 0 号： 0 ppb ； 1 号： 0.1 ppb ； 2 号： 0.3 ppb ； 3 号： 0.9 ppb ； 4 号： 2.7 ppb ； 5 号： 8.1 ppb

盐酸克仑特罗 -HRP 标记物 ······································································· 1 瓶 ( 6 mL)

20 ×浓缩洗涤液 ························································································· 1 瓶 (50 mL)

底物溶液 A ································································································· 1 瓶 (7 mL)

底物溶液 B ·································································································· 1 瓶 (7 mL)

终止液（含 2 M 的硫酸） ········································································· 1 瓶 (6 mL)

封板膜 ········································································································· 3 张

说明书 ········································································································· 1 份