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銆€銆€Huanglo ngbing 锛圚LB锛 is a severe disease in Citrus caused by the infection ofCandidatusLiberibacter asiaticus 锛圕Las锛夛紝 which has brought a bout substantial eco nomic losses in the global Citrus industry. Recently, HLB has been recognized as a plant immune-mediated disease resulting from theCLas-colo nization stimulated immune respo nses and the accumulation of excessive reactive oxygen species 锛圧OS锛 in Citrus tissues. Here, we report a manganese oxide nanozyme 锛圡ONPs锛 ba sed strategy to scavenge and regulate the ROS me tabolism in HLB infected Citrus, thereby protecting leaf tissues against oxidative stress. Transmission electron microscopy 锛圱EM锛 imaging revealed that Mo nPs enable efficient delivery into the intercellular space by spraying dispersion. ROS detection indicated the direct ROS scavenging ability of Mo nPs with high efficiency in HLB-Citrus with a bout 60% of ROS decreasing. Enzyme activation and gene ex pression analysis exhibited that the MONP treatment could regulate the ROS me tabolism gene in Citrus to alleviate oxidative stress. Current research demo nstrated that the HLB-Citrus sprayed with Mo nPs showed a noticeable protective effect within 22 days to alleviate the blotchy mottle symptoms. Furthermore, various physiological indexes such as MDA, starch, total soluble sugar, carotenoid and chlorophyll in HLB-Citrus leaves exhibited significant improvement post-MONP treatment. This MONP-ba sed approach provides a promising alternative strategy to modulate and mitigate oxidative stress in HLB-Citrus, thereby serving the Citrus industry.

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