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銆€銆€鍓嶆湡锛岃鍥㈤槦鍙戠幇SCMV渚垫煋寮曡捣鐨勫彲鍙樺壀鎺ヨ浆褰曟湰鐨勫彉鍖栧叿鏈変績杩涚梾姣掍镜鏌撶殑浣滅敤锛圖u et al., Plant Physiology, 2020, 184: 1514-1531锛夈€備负杩涗竴姝ョ悊瑙CMV璋冩帶鍙彉鍓帴鐨勬満鍒讹紝璇ョ爺绌朵腑鍒╃敤TurboID閭昏繎鏍囪缁撳悎娑茬浉鑹茶氨-涓茶仈璐ㄨ氨锛圠C-MS/MS锛夌殑鏂规硶锛岀瓫閫変簡澶氫釜涓嶯Ia-Pro娼滃湪浜掍綔鐨勫壀鎺ュ洜瀛愶紱杩涗竴姝ラ€氳繃鍙彉鍓帴鎶ュ憡绯荤粺銆佷簰浣滈獙璇佸強鍐呮簮鍓帴鍥犲瓙琛ㄨ揪娌夐粯瀹為獙锛岃瘉瀹炰簡SCMV缂栫爜鐨凬Ia-Pro閫氳繃涓嶼mU2AF65B鐨勪簰浣滐紝杩涜€屾搷绾礡NA鍓帴銆侟/div>
銆€銆€闅忓悗锛屽埄鐢↖so-Seq鎶€鏈紝璇ョ爺绌跺彂鐜癦mu2af65b鍔熻兘缂哄け绐佸彉浣擄紙d2锛変腑mRNA鐩戞祴閫氳矾鍩哄洜鐨勫彲鍙樺壀鎺ュ彂鐢熺磰涔憋紝涓旈澏鏍囪浆褰曟湰绱Н鏄庢樉锛屽垵姝ユ彮绀轰簡ZmU2AF65B閫氳繃鍙彉鍓帴姝ｈ皟鎺RNA鐩戞祴閫氳矾銆傞€氳繃涓嶴CMV渚垫煋鐨勭帀绫充腑鍙戠敓鍙彉鍓帴鍙樺寲鐨勮浆褰曟湰杩涜鑱斿悎鍒嗘瀽鍙戠幇锛屽湪SCMV渚垫煋鐨勭帀绫虫垨d2绐佸彉浣撲腑锛宮RNA鐩戞祴閫斿緞鐨?涓富瑕佸熀鍥狅紙鍖呮嫭ZmUPF3锛夌殑杞綍鏈湁绫讳技鐨勫彲鍙樺壀鎺ュ彉鍖栥€傚凡鏈夌爺绌惰〃鏄嶶PF3閫氳繃浣滅敤浜庡鏄惧瓙杩炴帴澶嶅悎鐗╋紙EJC锛夊湪mRNA鐩戞祴閫氳矾鐨勮捣濮嬮樁娈靛彂鎸ラ噸瑕佷綔鐢紝骞跺湪鍚庣画鐨凬MD鍒嗘敮涓綔涓烘牳蹇冪粍鍒嗭紱鎷熷崡鑺ヤ腑鐨凬MD閫斿緞閫氳繃闈跺悜AtUPF3浜х敓鐨勫叿鏈夐暱3'-UTR鐨勮浆褰曟湰鏉ヨ皟鑺侫tUPF3鐨刴RNA姘村钩锛屼粠鑰屼繚璇乵RNA鐩戞祴閫氳矾鐨勫姛鑳戒笉浼氳繃寮烘垨鑰呭お寮憋紙璐熷弽棣堝惊鐜級銆傛湁鎰忔€濈殑鏄紝鎴戜滑鍙戠幇鍦╓T鐜夌背涓紝ZmUPF3閫氳繃“鍐呭惈瀛愪繚鐣?rdquo;鑰屼骇鐢熷叿鏈夐暱3'-UTR鐨勮浆褰曟湰锛涗絾鍦╠2妞嶆牚涓笉浠呬骇鐢熸洿灏戠被鍨嬬殑ZmUPF3鍙彉鍓帴杞綍鏈紝鑰屼笖涓嶄骇鐢熷叿鏈夐暱3'-UTR鐨勮浆褰曟湰锛屼粠鑰岀牬鍧忎簡ZmUPF3鐨勮礋鍙嶉寰幆銆傞壌浜庝笂杩拌繖浜涢噸瑕佸彂鐜帮紝璇ョ爺绌朵箣鍚庤仛鐒﹀埌浜哯mUPF3銆傞€氳繃RNA-EMSA鍜孧ST绛夊疄楠岃瘉瀹炰簡ZmU2AF65B鍙互鐩存帴缁撳悎ZmUPF3鐨刾re-mRNA锛屼笖涓昏涓?‘鍓帴浣嶇偣闄勮繎鐨勫翱鍢у暥瀵岄泦涓茶繘琛岀粨鍚堬紝鑰屼笖瀵瑰翱鍢у暥瀵岄泦绋嬪害鏇撮珮鐨勫唴鍚瓙鐨勭粨鍚堣兘鍔涙洿寮猴紝杩涜€屼繚璇乑mUPF3杞綍鏈殑姝ｅ父鍙彉鍓帴妯″紡锛屽疄鐜板mRNA鐩戞祴閫氳矾鐨勬璋冩帶銆侟/div>
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鍔犲叆鏀惰棌] [
鍛婅瘔濂藉弸] [
鎵撳嵃鏈枃] [
鍏抽棴绐楀彛]
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涓浗鍐滀笟澶у妞嶄繚瀛﹂櫌鍗氬＋鍚庢潨寮€閫氾紙鍏ラ€夋垜鏍￠鎵瑰叴鍐滈潚骞村鑰咃級涓虹涓€浣滆€咃紝鍛ㄦ稕鏁欐巿涓洪€氳浣滆€咃紝妞嶇墿鐥呮瘨鐮旂┒瀹ゆ垚鍛樺崥澹敓褰緱鏅恒€佺帇鍩广€佸褰ゅ崥澹€侀檲鏇﹀崥澹拰鑼冨湪涓版暀鎺堬紝鑽峰叞鏍肩綏瀹佹牴澶у鍚村绉嬪崥澹紝鍗庡ぇ鍩哄洜鏈变簹鍏靛崥澹拰濮滀笁鏉板崥澹紝灞变笢鍐滀笟澶у鏉庡悜涓滄暀鎺堬紝娌冲寳鍐滀笟澶у鏇瑰織鑹虫暀鎺堜负鏈爺绌剁殑瀹屾垚鍋氬嚭浜嗛噸瑕佽础鐚€傚湪鏈枃鐮旂┒鍜屾挵鍐欒繃绋嬩腑寰楀埌浜嗘竻鍗庡ぇ瀛﹀垬鐜変箰鏁欐巿銆佸崡浜啘涓氬ぇ瀛﹁懀鑾庤悓鏁欐巿銆佷笂娴峰笀鑼冨ぇ瀛︿箶姘稿埄鏁欐巿銆佷腑鍥藉啘绉戦櫌妞嶄繚鎵€鏉庢柟鏂圭爺绌跺憳銆佷腑鍥藉啘涓氬ぇ瀛︾帇涔︿紵鍗氬＋銆佸紶姘镐寒鏁欐巿鍜岄儹娴烽緳鏁欐巿鐨勫ぇ鍔涘府鍔╁拰瀹濊吹寤鸿锛涜鐮旂┒寰楀埌浜嗗浗瀹惰嚜鐒剁瀛﹀熀閲戙€佷腑鍥藉崥澹悗绉戝鍩洪噾浼氬拰鍖椾含甯傜瀛﹀熀閲戠瓑鍩洪噾鐨勮祫鍔┿€侟/div>
銆€銆€鍘熸枃閾炬帴锛歨ttps://www.science.org/doi/10.1126/sciadv.adn3010
鏃ユ湡锛欬a href="//www.sqrdapp.com/news/2024-11-11.html">2024-11-11
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