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銆€銆€Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on a formulation of Bacillus subtilis DSM 33862 and Lentilactobacillus buchneri DSM 12856 when used as a technological additive to improve ensiling of fresh plant material. The additive is intended for use with fresh plant material with a dry matter (DM) range of 32%–65% at a proposed minimum co
ncentration of 1 × 10
8co
lony forming units (CFU)/kg fresh plant material to be used for all animal species. The bacterial species B. subtilis and L. buchneri are co
nsidered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment. The identity of the strains was established, no acquired antimicrobial resistance genes of co
ncern were detected in any of the two active agents and no evidence of toxigenic potential was found in B. subtilis DSM 33862. Therefore, the FEEDAP Panel co
ncluded that the use of the strains as a silage additive is co
nsidered safe for all the animal species, for co
nsumers of products from animals fed with the treated silage and for the environment. Regarding user safety, the additive co
ntains microorganisms and therefore should be co
nsidered as a potential skin and respiratory sensitiser. The additive is not irritant to skin or eyes. Inhalation and dermal exposure is co
nsidered a risk. These co
nclusions would apply, in principle, to any preparations co
ntaining the active agents. The FEEDAP Panel co
ncluded that the additive co
ntaining B. subtilis DSM 33862 and L. buchneri DSM 12856 at a minimum co
ncentration of 1 × 10
8CFU/kg fresh material has the potential to extend the aerobic stability of silage prepared from fresh plant materials with a DM range of 32%–65%.
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