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鍗庝腑鍐滀笟澶у瀛﹁€呭湪鏌戞鏋滃疄鍙剁豢绱犻檷瑙e拰绫昏儭钀濆崪绱犲悎鎴愬垎瀛愭満鍒剁爺绌朵腑鏈夋柊鍙戠幇

鏀惧ぇ瀛椾綋  缂╁皬瀛椾綋 鏃堕棿锛�2024-04-04 06:31 鏉ユ簮锛氬崕涓啘涓氬ぇ瀛� 鍘熸枃:
鏍稿績鎻愮ず锛氳鐮旂┒瑙f瀽浜嗚浆褰曞洜瀛怌rWRKY42閫氳繃鍗忓悓璋冩帶鍙剁豢绱犻檷瑙e拰绫昏儭钀濆崪绱犵敓鐗╁悎鎴愰€斿緞淇冭繘鏌戞鏋滃疄鐫€鑹茬殑鍒嗗瓙鏈哄埗锛岃鐮旂┒缁撴灉涓烘煈姗樻灉瀹炶壊娉藉搧璐ㄩ仐浼犳敼鑹彁渚涗簡鐞嗚渚濇嵁鍜岄噸瑕佸熀鍥犺祫婧愩€�
銆€銆€杩戞棩锛屽崕涓啘涓氬ぇ瀛﹀洯鑹烘灄瀛﹀闄�/鏋滆敩鍥壓浣滅墿绉嶈川鍒涙柊涓庡埄鐢ㄥ叏鍥介噸鐐瑰疄楠屽閭撶鏂伴櫌澹洟闃熷湪Plant Physiology鍦ㄧ嚎鍙戣〃浜嗛涓�“Transcription factor CrWRKY42 coregulates chlorophyll degradation and carotenoid biosynthesis in citrus”鐨勭爺绌惰鏂囥€傝鐮旂┒瑙f瀽浜嗚浆褰曞洜瀛怌rWRKY42閫氳繃鍗忓悓璋冩帶鍙剁豢绱�闄嶈В鍜岀被鑳¤悵鍗滅礌鐢熺墿鍚堟垚閫斿緞淇冭繘鏌戞鏋滃疄鐫€鑹茬殑鍒嗗瓙鏈哄埗锛岃鐮旂┒缁撴灉涓烘煈姗樻灉瀹炶壊娉藉搧璐ㄩ仐浼犳敼鑹彁渚涗簡鐞嗚渚濇嵁鍜岄噸瑕�鍩哄洜璧勬簮銆�
 
銆€銆€鏌戞鏄笘鐣屼笂鏈€閲嶈鐨勬灉鏍戜箣涓€锛屽叿鏈夊法澶х殑浜т笟鍜岀粡娴庝环鍊笺€傝繎骞存潵鎴戝浗鏌戞浜т笟蹇€熷彂灞曪紝绉嶆闈㈢Н鍜屼骇閲忓潎灞呬笘鐣岄浣嶏紝浜ч噺鍗犲埌涓栫晫鐨�1/3宸﹀彸銆傝壊娉芥槸鏌戞鏋滃疄鐨勯噸瑕佸搧璐ㄤ箣涓€锛屽彾缁跨礌鍜岀被鑳¤悵鍗滅礌缁勬垚鍙婂惈閲忓喅瀹氫簡鍏跺瑙傝壊娉姐€傚彾缁跨礌闄嶈В鍜岀被鑳¤悵鍗滅礌鐢熺墿鍚堟垚涓ゆ潯浠h阿閫斿緞鍗佸垎澶嶆潅锛屽彈澶氬熀鍥犲鑺傜偣澶氬洜绱犺皟鎺с€傚湪鏌戞鏋滃疄鎴愮啛杩囩▼涓紝鍙剁豢绱犻檷瑙e拰绫昏儭钀濆崪绱犵敓鐗╁悎鎴愬嚑涔庡悓鏃跺彂鐢燂紝鐒惰€岃繖涓や釜浠h阿杩囩▼鍦ㄨ浆褰曟按骞充笂鐨勫崗鍚岃皟鎺у皻涓嶅畬鍏ㄦ槑鏅般€�
 
銆€銆€鏈爺绌朵互“涓嶇煡鐏�”鍙婂叾榛勮壊鏋滃疄绐佸彉浣�“閲戜箰鏌�”涓烘潗鏂欐寲鎺樺埌涓€涓猈RKY瀹舵棌杞綍鍥犲瓙CrWRKY42鍙備笌鏌戞鏋滃疄杞壊杩涚▼銆傚湪鏌戞鎰堜激缁勭粐涓秴閲忚〃杈綜rWRKY42锛屽叓姘㈢暘鑼勭孩绱犺劚姘㈤叾锛圕rPDS锛夈€佺暘鑼勭孩绱�β-鐜寲閰讹紙CrLCYB2锛変互鍙�β-鑳¤悵鍗滅礌缇熷寲閰讹紙CrBCH1锛夌瓑澶氫釜绫昏儭钀濆崪绱犵敓鐗╁悎鎴愬熀鍥犲彈鍒拌瀵兼樉钁椾笂璋冭〃杈撅紝绫昏儭钀濆崪绱犲惈閲忔樉钁楀鍔犮€傚湪鏌戞鎰堜激缁勭粐涓共娑塁rWRKY42锛屾€荤被鑳¤悵鍗滅礌鍚噺鏄捐憲闄嶄綆锛屽涓被鑳¤悵鍗滅礌鍚堟垚鐩稿叧鍩哄洜鐨勮〃杈炬按骞冲彈鍒版姂鍒躲€傛澶栵紝鍦ㄦ煈姗樻灉瀹炰腑鐬椂瓒呴噺琛ㄨ揪鍜屽共娑塁rWRKY42鍩哄洜锛屽彂鐜癈rWRKY42鑳藉鍚屾椂婵€娲诲彾缁跨礌闄嶈В鍜岀被鑳¤悵鍗滅礌鐢熺墿鍚堟垚鍏抽敭鍩哄洜鐨勮〃杈撅紝鍗忓悓璋冩帶涓ゆ潯浠h阿閫斿緞锛岃繘鑰屼績杩涙煈姗樻灉瀹炶浆鑹层€傜敓鍖栧疄楠岀粨鏋滆〃鏄庯紝CrWRKY42鑳藉鐩存帴涓嶤rBCH1鍚姩瀛愪笂鐨刉-box鍏冧欢缁撳悎骞舵縺娲诲叾琛ㄨ揪銆傝繘涓€姝ョ爺绌跺彂鐜癈rWRKY42杩樿兘澶熺洿鎺ョ粨鍚堝苟婵€娲诲涓彾缁跨礌闄嶈В鍜岀被鑳¤悵鍗滅礌鐢熺墿鍚堟垚鍩哄洜鐨勫惎鍔ㄥ瓙锛屽寘鎷琋onYELLOW COLORING锛圕rNYC锛夈€丼TAY-GREEN锛圕rSGR锛夈€丆rPDS鍜孋rLCYB2銆�
 
銆€銆€浠ヤ笂缁撴灉琛ㄦ槑锛孋rWRKY42浣滀负鍙剁豢绱犻檷瑙e拰绫昏儭钀濆崪绱犵敓鐗╁悎鎴愮殑鐩存帴姝h皟鑺傚洜瀛愶紝鍦ㄦ煈姗樻灉瀹炵潃鑹茶繃绋嬩腑鍙戞尌閲嶈浣滅敤銆傝鐮旂┒缁撴灉鏈夊姪浜庢繁鍏ヨ璇嗘灉瀹炴垚鐔熻繃绋嬩腑鍙剁豢绱犲拰绫昏儭钀濆崪绱犱唬璋㈢殑澶嶆潅杞綍璋冩帶鏈哄埗锛屽浜庢灉瀹炶壊娉藉搧璐ㄦ敼鑹強璋冩帶鍏锋湁鎸囧鎰忎箟銆�
 
銆€銆€鍗庝腑鍐滀笟澶у鍥壓鏋楀瀛﹂櫌閭撶鏂伴櫌澹负璇ヨ鏂囩殑閫氳浣滆€咃紝鍗氬+鐢熼檲绾㈣壋涓鸿璁烘枃鐨勭涓€浣滆€呫€傚崕涓啘涓氬ぇ瀛︾澹敓绾儬瀹囥€佸崥澹敓榛勬枃閾犲拰寮犲摬鎯犮€佸崥澹悗鏈卞嚡鏉般€佸彾淇婁附鍓爺绌跺憳鍜屾煷鍒╁啗鏁欐巿浠ュ強涓浗鍐滀笟绉戝闄㈡煈妗旂爺绌舵墍鏈变笘骞冲壇鐮旂┒鍛樹篃鍙備笌浜嗘椤圭爺绌躲€傝鐮旂┒鍦ㄥ浗瀹堕噸鐐圭爺鍙戣鍒掑拰鍥藉鑷劧绉戝鍩洪噾鐨勮祫鍔╀笅瀹屾垚銆�
 
銆€銆€鑻辨枃鎽樿锛�
 
銆€銆€Chlorophyll degradation and carotenoid biosynthesis, which occur almost simultaneously during fruit ripening, are essential for the coloration and nutritional value of fruits. However, the synergistic regulation of these two processes at the transcriptional level remains largely unknown. Here, we identified a WRKY transcription factor, CrWRKY42, from the transcriptome data of the yellowish bud mutant 'Jinlegan' [锛圕itrus unshiu×C. sinensis锛�×C. reticulata] tangor 锛圡T锛� and its wild type 'Shiranuhi' tangor 锛圵T锛夛紝 which was involved in the transcriptional regulation of both chlorophyll degradation and carotenoid biosynthesis pathways. CrWRKY42 directly bound to the promoter of β-carotene hydroxylase 1 锛圕rBCH1锛� and activated its expression. Overexpression and interference of CrWRKY42 in citrus calli demonstrated that CrWRKY42 promoted carotenoid accumulation by inducing the expression of multiple carotenoid biosynthetic genes. Further assays confirmed that CrWRKY42 also directly bound to and activated the promoters of the genes involved in carotenoid biosynthesis, including phytoene desaturase 锛圕rPDS锛� and lycopene β-cyclase 2 锛圕rLCYB2锛夈€� In addition, CrWRKY42 could bind to the promoters of NonYELLOW COLORING 锛圕rNYC锛� and STAY-GREEN 锛圕rSGR锛� and activate their expression, thus promoting chlorophyll degradation. Overexpression and silencing of CrWRKY42 in citrus fruits indicated that CrWRKY42 positively regulated chlorophyll degradation and carotenoid biosynthesis by synergistically activating the expression of genes involved in both pathways. Our data revealed that CrWRKY42 acts as a positive regulator of chlorophyll degradation and carotenoid biosynthesis to alter the conversion of citrus fruit color. Our findings provide insight into the complex transcriptional regulation of chlorophyll and carotenoid metabolism during fruit ripening.
鏃ユ湡锛�2024-04-04
 

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