华中农业大学学者在甘蓝型油菜角果光合作用与种子产量形成机制研究中取得新进展

　　Trehalose-6-phosphate （T6P� functions as a vital proxy for assessing carbohydrate status in plants. While class II T6P synthases （TPS� do not exhibit TPS activity, they are believed to play pivotal regulatory roles in trehalose me tabolism. However, their precise functions in carbon me tabolism and crop yield have remained largely unknown. Here, BnaC02.TPS8, a class II TPS gene, is shown to be specifically expressed in mature leaves and the developing pod walls of Brassica napus. Over ex pression of BnaC02.TPS8 increased photosynthesis and the accumulation of sugars, starch, and biomass compared to wild type. me tabolomic analysis of BnaC02.TPS8 overexpressing lines and CRISPR/Cas9 mutants indicated that BnaC02.TPS8 enhanced the partitio ning of photoassimilate into starch and sucrose, as opposed to glycolytic intermediates and organic acids, which might be associated with TPS activity. Furthermore, the overex pression of BnaC02.TPS8 not o nly increased seed yield but also enhanced seed oil accumulation and improved the oil fatty acid composition in B. napus under both high nitrogen （N� and low N co nditions in the field. These results highlight the role of class II TPS in impacting photosynthesis and seed yield of B. napus, and BnaC02.TPS8 emerges as a promising target for improving B. napus seed yield.