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核心æ示:近日,åŽä¸­å†œä¸šå¤§å­¦å†œä¸šå¾®ç”Ÿç‰©èµ„æºæŒ–掘与利用全国é‡ç‚¹å®žéªŒå®¤æ¤ç‰©ç—…害生物防治团队æŽå›½åº†è¯¾é¢˜ç»„在Cell Reports上å‘表了研究论文。该研究å‘现在油èœèŠ±ç“£ä¸­ç‰¹å¼‚表达蛋白酶CmSp1å¯æ˜¾è‘—抑制死体è¥å…»åž‹ç—…原真èŒï¼ˆæ ¸ç›˜èŒå’Œç°è‘¡è„孢)的侵染,为油èœèŒæ ¸ç—…å’Œç°éœ‰ç—…的防控æ供了新策略ã€ü/div>
  近日,åŽä¸­å†œä¸šå¤§å­¦å†œä¸™üa href='//www.sqrdapp.com/news/tag_1901.html' class='zdbq' title='微生物相关食å“资è®? target='_blank'>微生ç‰?/a>资æºæŒ–掘与利用全国é‡ç‚¸üa href='//www.sqrdapp.com/news/tag_4793.html' class='zdbq' title='实验相关食å“资讯' target='_blank'>实验室æ¤ç‰©ç—…宲üa href='//www.sqrdapp.com/news/tag_4780.html' class='zdbq' title='生物相关食å“资讯' target='_blank'>生物防治团队æŽå›½åº†è¯¾é¢˜ç»„在Cell Reports上å‘表了题为¡°ex pression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens¡±çš„研究论文。该研究å‘现åœ?a href='//www.sqrdapp.com/news/tag_2263.html' class='zdbq' title='æ²¹èœç›¸å…³é£Ÿå“资讯' target='_blank'>æ²¹èœèŠ±ç“£ä¸­ç‰¹å¼‚表辽üa href='//www.sqrdapp.com/news/tag_294.html' class='zdbq' title='蛋白酶相关食å“资è®? target='_blank'>蛋白酵ü/a>CmSp1å¯æ˜¾è‘—抑制死体è¥å…»åž‹ç—…原 真èŒï¼ˆæ ¸ç›˜èŒå’Œç° è‘¡è„孢)的侵染,为油èœèŒæ ¸ç—…å’Œç°éœ‰ç—…的防控æ供了新策略ã€
  核盘èŒå¼•èµ·çš„æ²¹èœèŒæ ¸ç—…是我国油èœçš„首è¦ç—…害。该病å‘生范围广,å‘生é¢ç§¯å¤§ï¼Œæš´å‘频率高,å±å®³æŸå¤±é‡ï¼Œå·²æˆä¸ºæ²¹èœå®‰å…¨ç”Ÿäº§çš„主è¦é™åˆ¶å› å­ï¼Œä¸”对冬油èœäº§åŒºçš„æŸå®³å°¤å…¶ä¸¥é‡ã€‚由于缺ä¹æŠ—ç—…å“ç§ï¼ŒèŒæ ¸ç—…的防治主è¦ä¾èµ–于化学防治,但化学农è¯çš„长期使用(尤其是滥用)易导致抗è¯æ€§äº§ç”Ÿã€å†œè¯æ®‹ç•™å’ŒçŽ¯å¢ƒæ±¡æŸ“等问题。目å‰ï¼Œåœ¨å¸¸è§„防治æ¡ä»¶ä¸‹ï¼ŒèŒæ ¸ç—…造æˆçš„æ²¹èœç±½æŸå¤±æ¯å¹´çº¦ä¸º80亿元。因此,èŒæ ¸ç—…的绿色安全防控是油èœç”Ÿäº§ä¸ŠäºŸå¾…解决的难题ã€ü/div>
  核盘èŒåˆä¾µæŸ“æºå­å›Šå­¢å­çš„侵染能力较弱,需借助油èœèŠ±ç“£ï¼ˆå¤–æºè¥å…»ï¼‰æ‰èƒ½å½¢æˆä¾µæŸ“èŒä¸ï¼Œå®ƒä¸»è¦é€šè¿‡ç½¹ç—…花瓣飘è½è‡³èŒŽå¶å¼•å‘接触侵染的方å¼æ€æ­»æ¤æ ªã€‚因此,抑制å­å›Šå­¢å­ä¾µæŸ“花瓣是防治èŒæ ¸ç—…的有效策略。研究团队å‘现核盘èŒé‡å¯„生真èŒç›¾å£³éœ‰åˆ†æ³Œçš„蛋白酶CmSp1能é™è§£æ ¸ç›˜èŒç»†èƒžå£è›‹ç™½å’Œç»†èƒžè†œè›‹ç™½ï¼ŒæŠ‘制核盘èŒå­å›Šå­¢å­èŒå‘å’ŒèŒä¸ç”Ÿé•¿ï¼Œä¸”能显著抑制核盘èŒå­å›Šå­¢å­ä¾µæŸ“花瓣。基于此,团队使用花瓣特异å¯åŠ¨å­XY355在油èœèŠ±ç“£ä¸­ç‰¹å¼‚表达CmSp1,显著抑制核盘èŒå­å›Šå­¢å­å’Œç°è‘¡è„孢分生孢å­ä¾µæŸ“花瓣,从而切断了花瓣侵染介导的病害循环;åŒæ—¶ï¼ŒèŠ±ç“£è¡¨è¾¾CmSp1æ¤æ ªå¯¹æ²¹èœçš„生长å‘育ã€è§’果数ã€å•æ ªäº§é‡ã€åƒç²’é‡ã€å«æ²¹é‡å’Œè›‹ç™½å«é‡ç­‰å†œè‰ºæ€§çŠ¶å‡æ— æ˜¾è‘—å½±å“。这表明花瓣表达CmSp1在抗花瓣侵染病原èŒåˆ†å­è‚²ç§ä¸­å…·æœ‰é‡è¦çš„应用价值ã€ü/div>
  本研究以核盘èŒä¾µæŸ“花瓣为出å‘点,从生防èŒç›¾å£³éœ‰ä¸­ç­›é€‰ç”Ÿé˜²åŠŸèƒ½åŸºå› CmSp1,通过花瓣特异表达转基因技术创制抗病æ料,为花瓣侵染病害防控æ供了新的研究æ€è·¯ã€ü/div>
  我校农业微生物资æºæŒ–掘与利用全国é‡ç‚¹å®žéªŒå®¤ã€æ¹–北çœä½œç‰©ç—…害监测和安全控制é‡ç‚¹å®žéªŒå®¤åšå£«ç ”究生王永春为论文第一作者,æ¨é¾™æ•™æŽˆä¸ºè®ºæ–‡é€šè®¯ä½œè€…,课题组æŽå›½åº†æ•™æŽˆã€å´æ˜Žå¾·å‰¯æ•™æŽˆå’Œå¼ é™å‰¯æ•™æŽˆï¼ŒåŠ æ‹¿å¤§è¨æ–¯å–€å½»æ¸©å¤§å­¦å«æ‰¬æ–—教授ã€ç¾Žå›½åŽç››é¡¿å·žç«‹å¤§å­¦é™ˆå«ä¸œæ•™æŽˆã€ä¸­å›½å†œç§‘院油料作物研究所刘胜毅研究员ã€å†œä¸šå¾®ç”Ÿç‰©èµ„æºæŒ–掘与利用全国é‡ç‚¹å®žéªŒå®¤å§œé“å®æ•™æŽˆå’ŒKenichi Tsuda教授等å‚与了åˆä½œç ”究。研究得到国家自然科学基金和国家油èœäº§ä¸šæŠ€æœ¯ä½“系(CARS-12)等项目的资助ã€ü/div>
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  Sclerotinia sclerotiorum and Botrytis cinerea are necrotrophic plant-pathogenic fungi, causing substantial eco nomic losses on many crops. So far, resistant cultivars against these pathogens are unavailable in most crops. Here, we show that the serine protease CmSp1 of Co niothyrium minitans, a well-characterized mycoparasite of S. sclerotiorum, co ntributed to suppressing the petal-mediated infection by S. sclerotiorum in rapeseed. Application of recombinant CmSp1 proteins facilitates the bulk degradation of S. sclerotiorum proteins and inhibits spore germination and hyphal growth of S. sclerotiorum and B. cinerea, thereby preventing the development of both diseases. Stable transgenic rapeseed plants with tissue-specific ex pression of CmSp1 in flower petals inhibit the petal-mediated infection by both S. sclerotiorum and B. cinerea, and resulting transgenic plants have no adverse effect on other agro nomic traits. Thus, our findings provide a novel mechanism by which a mycoparasite inhibits fungal pathogens and an enviro nmentally friendly disease management strategy.
  论文链接:https://www.sciencedirect.com/science/article/pii/S2211124723013025
日期9üa href="//www.sqrdapp.com/news/2023-11-07.html">2023-11-07
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