华中农业大学在国际期刊发表微生物修复稻田土壤锑污染的研究成果

　　Antimony （Sb� is toxic to ecosystems and potentially to public health via its accumulation in the food chain. Bioavailability and toxicity of Sb have been reduced using various methods for the remediation of Sb-co ntaminated soil in most studies. However, Sb-co ntaminated soil remediation by microbial agents has been rarely e valuated. In this study, we e valuated the potential for the use of Comamo nas testosteroni JL40 in the bioremediation of Sb-contamination. Strain JL40 immobilized more than 30% of the Sb（III� in solution and oxidized over 18% to Sb（V� for detoxification. Meanwhile, strain JL40 respo nds to Sb toxicity through such as Sb efflux, intracellular accumulation, biofilm production, and scavenging of reactive oxygen species （ROS）， etc. The results of the pot experiment showed the average Sb co ntent of the brown rice was decreased by 59.1%, 38.8%, and 48.4%, for 1.8, 50, and 100 mg/kg Sb spiked soils, respectively. In addition, the results of plant, soil enzyme activity, and rice agro nomic trait observations showed that the application of strain JL40 could maintain the health of plants and soil and improve rice production. The single-step and sequential extraction of Sb from rhizosphere soil showed that strain JL40 also plays a role in Sb immobilization and oxidation in the soil environment. During rice potted cultivation, bacterial community analysis and plate counting showed that the strain JL40 could still maintain 103 CFU/g after 30 days of inoculation. With phenotypic and differential proteomics analysis, strain JL40 co nferred Sb（III� tolerance by a combination of immobilization, oxidation, efflux and scavenging of ROS, etc. Our study demo nstrates the application of Sb-immobilizing and oxidizing bacteria to lower soil Sb and reduce accumulation of Sb in rice. Our results provide guidance for bacterial remediation of Sb-co ntaminated soil.

　　论文链接：https://doi.org/10.1016/j.envint.2023.108040