华中农业大学油菜团队揭示lncRNAs影响油菜种子含油量积累的潜在机制

　　Background

　　Studies have indicated that long non-coding RNAs （lncRNAs� play im portant regulatory roles in many biological processes. However, the regulation of seed oil biosynthesis by lncRNAs remains largely unknown.

　　Results

　　We comprehensively identified and characterized the lncRNAs from seeds in three developing stages in two accessions of Brassica napus （B. napus）， ZS11 （high oil content� and WH5557 （low oil content）� Finally, 8094 expressed lncRNAs were identified. LncRNAs MSTRG.22563 and MSTRG.86004 were predicted to be related to seed oil accumulation. Experimental results show that the seed oil co ntent is decreased by 3.1�C3.9% in MSTRG.22563 overex pression plants, while increased a bout 2% in MSTRG.86004, compared to WT. Further study showed that most genes related to lipid me tabolism had much lower ex pression, and the co ntent of some me tabolites in the processes of respiration and TCA （tricarboxylic acid� cycle was reduced in MSTRG.22563 transgenic seeds. The ex pression of genes involved in fatty acid synthesis and seed embryo nic development （e.g., LEC1� was increased, but genes related to TAG assembly was decreased in MSTRG.86004 transgenic seeds.

　　Conclusion

　　Our results suggest that MSTRG.22563 might impact seed oil co ntent by affecting the respiration and TCA cycle, while MSTRG.86004 plays a role in prolo nging the seed developmental time to increase seed oil accumulation.

　　论文链接：https://doi.org/10.1186/s13068-022-02256-1