銆€銆€2022骞�11鏈�3鏃ワ紝妞嶇墿淇濇姢瀛﹂櫌妞嶇墿鍏嶇柅鐮旂┒鍥㈤槦鍦ㄥ浗闄呰憲鍚嶅鏈湡鍒奝lant Biotechnology Journal涓婂湪绾垮彂琛ㄤ簡棰樹负“Simultaneous editing of three homoeologs of TaCIPK14 confers broad-spectrum resistance to stripe rust in wheat”鐨勭爺绌惰鏂囥€傝鐮旂┒閴村畾鍒颁竴涓劅鏉¢攬鐥呮縺閰�鍩哄洜TaCIPK14锛屽苟鍒╃敤CRISPR/Cas9鍩哄洜缂栬緫鎶€鏈垱鍒朵簡灏忛害骞胯氨鎶楃梾鏂版潗鏂欍€傛鐗╀繚鎶ゅ闄㈤儹鍐涙暀鎺堝拰搴锋尟鐢熼櫌澹负鏈枃鐨勯€氳浣滆€咃紝鍗氬+鐮旂┒鐢熶綍浠樻柊涓鸿鏂囩殑绗竴浣滆€呫€�
銆€銆€鐢辨潯褰㈡焺閿堣弻灏忛害涓撳寲鍨嬶紙Puccinia striiformis f. sp. tritici, Pst锛夊紩璧风殑鏉¢攬鐥呮槸鎴戝浗灏忛害鐢熶骇涓婃渶閲嶈鐥呭涔嬩竴銆傜妞嶅拰鍩硅偛鎶楃梾鍝佺鏄槻娌诲皬楹︽潯閿堢梾鏈€缁忔祹鏈夋晥鐨勬帾鏂姐€傜劧鑰岋紝鐢变簬瀛樺湪灏忛害鎶楃梾璧勬簮涓嶈冻鍙婂搧绉嶅瀭鐩存姉鎬ф槗“涓уけ”绛夐棶棰橈紝瀵艰嚧浼犵粺鎶楅攬鑲茬闈复宸ㄥぇ鎸戞垬銆傚洜姝わ紝浜熼渶鎸栨帢鏂扮殑鍩哄洜璧勬簮锛屽紑鍙戞柊鍨嬫姉鐥呯瓥鐣ワ紝鏀硅壇灏忛害鎶楃梾鎬т互瀹炵幇灏忛害鏉¢攬鐥呯殑鎸佷箙鎺у埗銆傛劅鐥呭熀鍥犻€氳繃淇冭繘鐥呭師鑿岃瘑鍒€佷镜鍏ュ瘎涓绘垨璐熻皟鎺ф鐗╁厤鐤弽搴斿彂鎸ユ劅鐥呯殑鍔熻兘銆傞€氳繃鍩哄洜缂栬緫鎶€鏈獊鍙樻劅鐥呭熀鍥犳槸鏀硅壇妞嶇墿鎶楃梾鎬х殑楂樻晥鏂伴€斿緞锛屽凡鎴愪负褰撲粖鍥介檯妞嶇墿鐥呯悊鐣岀殑鐮旂┒鐑偣銆�
銆€銆€绫婚挋璋冪缁忕7閰搁叾B鏍疯泲鐧斤紙Calcineurin B-like proteins锛孋BLs锛夌粨鍚堢殑铔嬬櫧婵€閰讹紙CBL-interacting protein kinases锛孋IPK锛夊弬涓庤皟鑺傛鐗╅挋淇″彿渚濊禆鐨勫悇绉嶇敓鐞嗚繃绋嬨€傚厛鍓嶇爺绌惰〃鏄庯紝姘寸ɑ涓璒sCIPK14/15涓哄€嶅鍩哄洜锛屽苟涓旀璋冩帶寰敓鐗╃浉鍏冲垎瀛愭ā寮忚瀵肩殑闃插尽鍙嶅簲銆傚簭鍒楀垎鏋愬彂鐜帮紝涓庢按绋籓sCIPK14/15涓嶅悓锛屽皬楹︾殑鍚屾簮铔嬬櫧TaCIPK14鍜孴aCIPK15鍑虹幇搴忓垪鍒嗗寲锛屾殫绀篢aCIPK14鍜孴aCIPK15瀛樺湪鍔熻兘鍒嗗寲銆傜灛鏃舵矇榛楾aCIPK14鍚庯紝灏忛害瀵规潯閿堣弻鐨勬姉鐥呮€ф樉钁楁彁楂橈紝浼撮殢鐫€浜у鍑忓皯銆丠2O2绉疮澧炲姞鍜岀梾绋嬬浉鍏冲熀鍥犳樉钁椾笂璋冭〃杈俱€傝繘涓€姝ュ垱鍒朵簡TaCIPK14鐨勮浆鍩哄洜杩囪〃杈炬潗鏂欙紝鎺ョ鍚庡彂鐜拌鏉愭枡瀵规潯閿堣弻鎰熺梾鎬у鍔狅紝涓昏琛ㄧ幇鍦ㄤ骇瀛㈠鍔狅紝娲绘€ф哀绉疮鍑忓皯鍜岀梾绋嬬浉鍏冲熀鍥犺〃杈捐鏄捐憲鎶戝埗銆傜患涓婅〃鏄嶵aCIPK14鏄皬楹︿腑涓€涓劅鏉¢攬鐥呭熀鍥犮€�
銆€銆€涓轰簡杩涗竴姝ユ槑纭甌aCIPK14鐨勫姛鑳藉強鍏跺簲鐢ㄦ綔鍔涳紝鏈爺绌跺埄鐢–RISPR/Cas9鍩哄洜缁勭紪杈戞妧鏈湪鍏€嶄綋灏忛害涓悓鏃舵暡闄や簡A銆丅銆丏鍩哄洜缁勭殑TaCIPK14鍩哄洜銆俆aCIPK14绐佸彉鏍郴瀵瑰寘鎷腑鍥藉綋鍓嶆潯閿堣弻娴佽灏忕CYR32銆丆YR33鍜孋YR34鍦ㄥ唴鐨�5涓潯閿堣弻灏忕浜х敓浜嗗箍璋辨姉鎬с€傜敯闂磋瘯楠岀粨鏋滆〃鏄庯紝TaCIPK14绐佸彉鏍郴涓庡鐓ф牚绯荤浉姣斾骇閲忕浉鍏冲啘鑹烘€х姸鏃犳樉钁楀樊寮傦紝鎺ョ鍚嶵aCIPK14绐佸彉鏍郴鐨勬潯閿堢梾涓ラ噸搴︿粠瀵圭収鏍郴鐨�64.5锛呴檷浣庡埌51.5锛呫€�
銆€銆€缁间笂锛岃鐮旂┒鍒╃敤鍩哄洜缂栬緫鎶€鏈獊鍙樺皬楹︽劅鏉¢攬鐥呭熀鍥燭aCIPK14锛屽垱鍒跺嚭骞胯氨鎶楃梾鏉愭枡锛屽湪涓嶅奖鍝嶄骇閲忔€х姸鐨勫墠鎻愪笅璧嬩簣浜嗗皬楹﹀鏉¢攬鑿岀殑骞胯氨鎶楁€э紝涓轰綔鐗╂姉鐥呮敼鑹彁渚涗簡鏂版€濊矾銆�
銆€銆€璇ョ爺绌跺緱鍒颁簡鏃卞尯浣滅墿閫嗗鐢熺墿瀛﹀浗瀹堕噸鐐瑰疄楠屽瀹為獙骞冲彴鎻愪緵鐨勬妧鏈敮鎸併€傚苟寰楀埌鍥藉閲嶇偣鐮斿彂璁″垝锛�2021YFD1401000锛夛紝鍥藉鑷劧绉戝鍩洪噾椤圭洰锛�32172381鍜�31972224锛夛紝闄曡タ鐪侀噸鐐圭爺鍙戣鍒掞紙2021ZDLNY01-01锛夛紝闄曡タ鐪佽嚜鐒剁瀛﹀熀纭€鐮旂┒鍙戝睍璁″垝锛�2020JZ-13锛夊拰鏁欒偛閮�111椤圭洰锛圔07049锛夎祫鍔┿€�
銆€銆€鍘熸枃閾炬帴锛歨ttps://onlinelibrary.wiley.com/doi/10.1111/pbi.13956