华中农业大学园艺与城市昆虫学团队在橘小实蝇代谢稳态的调控机制研究中取得新进展

　　Increasing evidence indicates that miRNAs play crucial regulatory roles in various physiological processes of insects, including systemic me tabolism. However, the molecular mechanisms of how specific miRNAs regulate energy me tabolic homeostasis remain largely unknown. In the present study, we found that an evolutio narily co nserved miR-275/305 cluster was essential for maintaining energy me tabolic homeostasis in respo nse to dietary yeast stimulation in Bactrocera dorsalis. Depletion of miR-275 and miR-305 by the CRISPR/Cas9 system significantly reduced triglyceride and glycogen contents, elevated total sugar levels, and impaired flight capacity. Combined in vivo and in vitro experiments, we demo nstrated that miR-275 and miR-305 can bind to the 3'UTR regions of SLC2A1 and GLIS2 to repress their ex pression, respectively. RNAi-mediated knockdown of these two genes partially rescued me tabolic phenotypes caused by inhibiting miR-275 and miR-305. Furthermore, we further illustrated that the miR-275/305 cluster acting as a regulator of the me tabolic axis was co ntrolled by the insulin signaling pathway. In conclusion, our work combined genetic and physiological approaches to clarify the molecular mechanism of me tabolic homeostasis in respo nse to different dietary stimulations and provided a reference for deciphering the potential targets of physiologically im portant miRNAs in a non-model organism.

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