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銆€銆€璇ョ爺绌朵互“Genomic basis underlying the metabolome-mediated drought adaptation of maize”涓洪鍦ㄥ浗闄呭鏈湡鍒� Genome Biology涓婂彂琛ㄣ€�

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銆€銆€鎴戞牎浣滅墿閬椾紶鏀硅壇鍥藉閲嶇偣瀹為獙瀹ょ殑寮犳枑鍜屽惔閿﹀嘲鍗氬＋鐢熶负璇ヨ鏂囩殑鍏卞悓绗竴浣滆€呫€備唬鏄庣悆鏁欐巿锛岄檲浼熸暀鎺堝拰浠ヨ壊鍒楁湰·鍙ゅ痉閲屽畨澶у鐨刌ariv Brotman 鏁欐巿涓鸿璁烘枃鐨勫叡鍚岄€氳浣滆€呫€傛湰鐮旂┒寰楀埌浜嗘姹夊競搴旂敤鍩虹鍓嶆部椤圭洰銆佸寳浜競鏉板嚭绉戝瀹堕」鐩拰鍩洪噾濮斿浗闄咃紙鍦板尯锛夊悎浣滀笌浜ゆ祦椤圭洰鐨勪富瑕佽祫鍔┿€�

 

銆€銆€鎹簡瑙ｏ紝鎴戞牎浠ｆ槑鐞冭棰樼粍澶氬勾鏉ュ洿缁曠帀绫虫姉鏃卞熀鍥犲厠闅嗐€佹姉鏃遍仐浼犳満鍒惰В鏋愬拰浼樿壇鎶楁棻绛変綅鍙樺紓鎸栨帢绛夋柟鍚戣繘琛屾繁鍏ョ爺绌讹紝宸插彇寰楀椤规垚鏋滐紝鍦ㄥ寘鎷珿enome Biology, PNAS, Molecular plant, Plant Journal绛塖CI鏉傚織鍙戣〃澶氱瘒璁烘枃銆備粖骞�6鏈堜唤锛屼唬鏄庣悆璇鹃缁勪富瀵煎悎浣滃湪Genome Biology鍙戞枃锛屾姤閬撲簡鍩轰簬楂橀€氶噺琛ㄥ瀷缁勬妧鏈紝缁撳悎鍙樺紓缁勩€佽〃杈剧粍鍜屽垎瀛愮敓鍖栨墜娈垫彮绀虹帀绫虫姉鏃遍仐浼犱笌鍒嗗瓙鏈哄埗鐨勭爺绌讹紙Wu et al., 2021锛夈€傛湰璁烘枃鏄璇鹃缁勫湪Genome Biology鏉傚織鐨勫啀娆＄爺绌舵姤閬撱€傚熀浜庡缁勫鍜屽垎瀛愮敓鍖栨墜娈佃仈鍚堝垎鏋愶紝浠ｆ槑鐞冭棰樼粍绯荤粺鍦版彮绀轰簡鐜夌背澶ц妯＄兢浣撳湪骞叉棻鑳佽揩鏉′欢涓嬬殑澶栧湪锛堝鏍瀷銆佸彾鑹层€佺敓鐗╅噸銆佸瓨娲荤巼绛夛級鍜屽唴鍦紙濡傞珮鍏夎氨鏁板瓧琛ㄥ瀷銆佹牳纾佸叡鎸唴閮ㄧ粨鏋勩€佷唬璋㈢墿绛夛級琛ㄥ瀷鍙樺寲锛屽苟鍩轰簬杩欎簺涓板瘜鐨勮〃鍨嬪彉寮傛瘮杈冨叏闈㈠湴闃愭槑浜嗙帀绫崇殑鎶楁棻閬椾紶鏈哄埗锛屾彮绀轰簡鍖呮嫭ZmcPGM2, ZmFAB1A, ZmBx12鍜孼mGLK44鍦ㄥ唴鐨勪竴鎵规柊鐨勩€侀噸瑕佺殑鍊欓€夊熀鍥犵殑鎶楁棻鍔熻兘銆佷綔鐢ㄦ満鐞嗭紝涓轰績杩涙垜鍥界帀绫虫姉鏃辫偛绉嶈繘绋嬫墦涓嬩簡鑹ソ鐨勫熀纭€銆�

 

銆€銆€鍘熸枃閾炬帴锛歨ttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-021-02481-1

 

銆€銆€鑻辨枃鎽樿锛�

 

銆€銆€Background

 

銆€銆€Drought is a major environmental disaster that causes crop yield loss worldwide. metabolites are involved in various environmental stress responses of plants. However, the genetic control of metabolomes underlying crop environmental stress adaptation remains elusive.

 

銆€銆€Results

 

銆€銆€Here, we perform non-targeted metabolic profiling of leaves for 385 maize natural inbred lines grown under well-watered as well as drought-stressed conditions. A total of 3890 metabolites are identified and 1035 of these are differentially produced between well-watered and drought-stressed conditions, representing effective indicators of maize drought response and tolerance. Genetic dissections reveal the associations between these metabolites and thousands of single-nucleotide polymorphisms 锛圫NPs锛夛紝 which represented 3415 metabolite quantitative trait loci 锛坢QTLs锛� and 2589 candidate genes. 78.6% of mQTLs 锛�2684/3415锛� are novel drought-responsive QTLs. The regulatory variants that control the expression of the candidate genes are revealed by expression QTL 锛坋QTL锛� analysis of the transcriptomes of leaves from 197 maize natural inbred lines. Integrated metabolic and transcriptomic assays identify dozens of environment-specific hub genes and their gene-metabolite regulatory networks. Comprehensive genetic and molecular studies reveal the roles and mechanisms of two hub genes, Bx12 and ZmGLK44, in regulating maize metabolite biosynthesis and drought tolerance.

 

銆€銆€Conclusion

 

銆€銆€Our studies reveal the first population-level metabolomes in crop drought response and uncover the natural variations and genetic control of these metabolomes underlying crop drought adaptation, demonstrating that multi-omics is a powerful strategy to dissect the genetic mechanisms of crop complex traits.