近年的科研项目

 

　　1. 国家自然科学基金项目（31672244）：刺激隐核虫诱发宿主产生免疫保护反应的分子筛选，在研，主持。

 

　　2. 国家重点研发计划政府间国际科技创新合作重点专项（2016YFE0121900）：PD-L1  KRAS 信号通路相关性及其临床应用，在研，参与。

 

　　3. 国家自然科学基金项目（31040084）：刺激隐核虫的表面抗原和胸苷激酶的鉴定和特征分析，已结题，主持。

 

　　4. 福建省自然科学基金重点项目：海洋鱼类寄生纤毛虫的流行病学调查与分子免疫学分析，已结题，主持。

 

　　5. 福建省自然科学基金项目：长角血蜱谷氨酰胺转胺酶的生物功能及应用价值探讨，主持。

 

　　6. 福建省科技厅JK项目：重组刺激隐核虫14-3-3蛋白在刺激隐核虫免疫诊断和预防中的应用价值探讨，已结题，主持。

 

　　7. 福建省自然科学基金项目：微小牛蜱半乳糖凝集素的鉴定和特征分析，已结题，参与。

 

　　8. 福建省重大专项子专题：福建省养殖大黄鱼刺激隐核虫病综合防控技术研究与示范，参与。

　　已发表的主要论文（* 为通讯作者）

 

　　1.Lin Q, M.Yang, Z. Huang, W. Ni, G. Fu, Z. Wang, X. Huang*. Cloning, expression and molecular characterization of a 14-3-3 gene from a parasitic ciliate, Cryptocaryon irritans. Veterinary parasitology. 197(3-4):427-35. 2013.

 

　　2.Huang X*, Y. Xu, G. Guo, Q. Lin, Z. Ye, L. Yuan, Z. Sun, W. Ni. Molecular characterisation of an actin depolymerising factor from Cryptocaryon irritans. Parasitology. 140 (5): 561-568. 2013.

 

　　3.Huang X*, Z. Sun, G. Guo, C. Zheng, Y. Xu, L. Yuan, C. Liu. Cloning and molecular characterization of a surface antigen from Cryptocaryon irritans. Experimental parasitology. 130 (3): 189-194. 2012.

 

　　4.Zhang, G., X. Huang, D. Boldbaatar, B. Battur, B. Battsetseg, H. Zhang, L. Yu, Y. Li, Y. Luo, S. Cao, Y. Goo, J. Yamagishi, J. Zhou, S. Zhang, H. Suzuki, I. Igarashi, T. Mikami, Y. Nishikawa, X. Xuan. Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice. Vaccine. 28: 7243–7247. 2010.

 

　　5.M.K. Islam, N. Tsuji, T. Miyoshi, M.A. Alim, X. Huang, T. Hatta, K. Fujisaki. The Kunitz-like modulatory protein haenangin is vital for hard tick blood feeding success. Plos Pathog. 5 (7). e1000497, 2009.

 

　　6.Huang X., N. Tsuji, T. Miyoshi, S. Nakamura-Tsuruta, J. Hirabayashi, and K. Fujisaki.. Molecular characterization and oligosaccharide binding properties of a galectin from the argasid tick Ornithodoros moubata. Glycobiology. 17(3): 313-323, 2007.

 

　　7.Huang X., N. Tsuji, T. Miyoshi, M. Motobu, M.K. Islam, M.A. Alim, and K. Fujisaki. Characterization of glutamine: fructose-6-phosphate aminotransferase from the ixodid tick, Haemaphysalis longicornis, and its critical role for host blood feeding. International Journal of Parasitology. 37(3-4): 383-392, 2007.

 

　　8.Abdul Alim M, N. Tsuji, T. Miyoshi, M. K. Islam, X. Huang, M. Motobu, K. Fujisaki. Characterization of asparaginyl endopeptidase, legumain induced by blood feeding in the ixodid tick Haemaphysalis longicornis.Insect Biochem Mol Biol. 37(9):911-22, 2007.

 

　　9.Motobu M., N. Tsuji, T. Miyoshi, X. Huang, M. K. Islam, M. A. Alim, K. Fujisaki. Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis.FEBS J. 274(13):3299-312, 2007.

 

　　10.Huang X., X. Xuan, R.A. Verdida, S. Zhang, N. Yokoyama, L. Xu, and I. Igarashi. Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses. Clinical Vaccine Immunology. 13(5): 553-555, 2006.

 

　　11.Huang X., X. Xuan, N. Yokoyama, Y. Katayama, T. Anzai, and I. Igarashi. evaluation of enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infections. Veterinary Parasitology. 140(1-2): 158-161, 2006.

 

　　12.Huang, X., X. Xuan, L. Xu, S. Zhang, N. Yokoyama, N. Suzuki, and I. Igarashi. Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses. Journal of Clinical Microbiology. 42: 359-361, 2004.

 

　　13.Huang, X., X. Xuan, H. Hirata, N. Yokoyama, L. Xu, N. Suzuki,and I. Igarashi. Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats. Journal of Clinical Microbiology. 42: 351-353, 2004.

 

　　14.Kumar, S, N. Yokoyama, J. Kim, X. Huang, N. Inoue, X. Xuan, I. Igarashi, and C. Sugimoto. expression of Babesia equi EMA-1 and EMA-2 during merozoite developmental stages in erythrocyte and their interaction with erythrocytic membrane skeleton. Molecular and Biochemical Parasitology. 133: 221-227, 2004.

 

　　15.Huang, X., X. Xuan, N. Yokoyama, L. Xu, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, and I. Igarashi. High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential in immunodiagnosis. Journal of Clinical Microbiology. 41: 1147-1151, 2003.

 

　　16. Huang X, X. Xuan, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, T. Mikami, and I. Igarashi. Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay. Clinical Diagnostic Laboratory Immunology. 9: 1343-1347, 2002.