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173.140 Esterase-lipase derived from Mucor miehei.
Esterase-lipase enzyme, consisting of enzyme derived from Mucor miehei var. Cooney et Emerson by a pure culture fermentation process, with maltodextrin or sweet whey as a carrier, may be safely used in food in accordance with the following conditions:
（a Mucor miehei var. Cooney et Emerson is classified as follows: Class, Phycomycetes; subclass, Zygomycetes; order, Mucorales; family, Mucoraceae; genus, Mucor; species, miehei; variety Cooney et Emerson.
（b The strain of Mucor miehei var. Cooney et Emerson is nonpathogenic and nontoxic in man or other animals.
（c The enzyme is produced by a process which completely removes the organism Mucor miehei var. Cooney et Emerson from the esterase-lipase.
（d The enzyme is used as a flavor enhancer as defined in 170.3（o）（12?
（e The enzyme is used at levels not to exceed current good manufacturing practice in the following food categories: cheeses as defined in 170.3（n）（5 of this chapter; fat and oils as defined in 170.?）（n）（12 of this chapter; and milk products as defined in 170.?）（n）（31 of this chapter. Use of this food ingredient is limited to nonstandarized foods and those foods for which the relevant standards of identity permit such use.
（f The enzyme is used in the minimum amount required to produce its limited technical effect.
[47 FR 28090, June 29, 1982; 48 FR 2748, Jan. 21, 1983]
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