Following a request for technical assistance, EFSA carried out an exposure assessment of the colouring principles bixin and norbixin of the food colour annatto extracts （E 160b）， from its use as a food additive, taking into account new proposed uses and use levels. In 2016, the EFSA Panel on Food Additives and Nutrient Sources added to Food （ANS� adopted a scientific opinion on the safety of annatto extracts （E 160b� as a food additive. In that opinion, the Panel co ncluded that the toxicological databa se was sufficient to derive an acceptable daily intake （ADI� of 6 mg bixin/kg body weight （bw� per day and an ADI of 0.3 mg norbixin/kg bw per day.

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