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Sketch the elution profile expected if this mixture is run on a Sephadex G-100 gel filtration column run in 50 mM phosphate buffer, pH 6. Label the peaks. b. Sketch the gel obtained from running these proteins on an SDS polyacrylamide electrophoresis gel at pH 8.0, (after staining and destaining). c. What predictions can you make about the results of a native PAGE at pH 7.6 ( state any assumptions you might need to make about the % acrylamide in the gel ) Z  d. Sketch the elution profile of these proteins from a carboxymethyl cellulose ion exchange chromatography column, run at pH 6.25 (with a salt gradient, if necessary). Label the peaks.   ,{N ˆ}v(Sm^vKm[ $ "$ RQl[.ll S)2l ygFolin)-ZՋBRl le݄grl +}YIQ8T6el **  Determination of enzyme activity!!B( !Enzyme activity v;mR v;m'` Active unitvv;mRUSMO Specific activityvvk;mR IZIB<    Enzyme activity@ [IN cvPSN[Sf[S^vR (u(WN[agN N v@bPSvgNSf[S^^R^ egh:y el Km[NirXRvϑb^irQ\vϑ eg. Sf[n[0kr0Km[+}Y8T6e0gIQKm[ B`0Z)ae4aG Active unit U @( DVEUSMO 1*Nv;mRUSMO /fc(Wyr[agN N (W1RQlS1_id\^irvvϑ b/flS^ir-N1_id\v gsQWVvvϑ0 yr[agN)n^ 25oC vQNagNeg. pHS^irSm^ GWǑ(ugagN0 `N`(ul eg. a-m|v (uk\ePS1gSn'`m|mS@bvvϑegh:y0ZacBai:aa  [O ja-m|v b-m|v gv ˆ}vv v Ǐ'lSirv (POD) YZ'lSv (PPO) 'lTv .6/dc0  ( a-m|v;mRKm[el x>frR NMv^ ^ir|^ NM^ |.Sbev^ b-m|v;mRvKm[el |b_bv^ 3 5-N]xW4lhgx00lSbx'`ܔvnmKm[؏SWVb_bv^ .c)8Z)gv;mRvKm[ PE (pectin esterasegov) n[l(pH-statl) PG (polygalacturonaseZJSsN|xv)TPMG (polymethylgalacturonaseZ2uWJSsN|xv):NQRve |^l PMGL polymethylgalaturomide lyaseZ2uWJSsN|xˆv PGL polygalacturonmide lyaseZJSsN|xˆv M㉧NirvC4TC5KNb_bS. 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Ǐ'lSirv;mRvKm[ ! ^St g"lOSOX[(WvagN N Ǐ'lSirvPSǏ'lS"lR㉄v Teb_b grSTir0 elRIQIQ^l00! 0YZ'lSv;mRvKm[ &Km[^irm1Yv^ Km[Nirubv^  'lTvv;mRKm[  ϑSl RIQIQ^l  !Specific activity@ rk;mR kkKQˆ}v@bwQ gvv;mR USMO USMO/kKQˆ}v U/mgˆ}v( [ TNyv k;mRaؚ va~v)ZZZacacg2"  #  $%&,Complete the following on purification table-- , c0, /8'(H0 0` TTrff` TTrh-[ʬ` 333MMM` TTr8r-[ffV>` TTrQ[-[` TTrTz<7FӥaG|` TTr4eXQƄΗ|aHb>?" dd@,? " Zd@  P " F@ ` n?" dd@   @@``PR    @ ` ` p>>  ++!!r*(  X  C @ABCs  # @ABCE@F||Y   #@`S"    @ABCDEdF&GshV #.9@Rdv~\B$ @ `"b_  S ,@ABCDEF 2||"" 'nHDi7nfK5$<Da-OBWb~f 3uYVF3 @  S"8b  S @ABCDEtF|| 1KgeM, "+.=Lgl|WG6@ S" ~4  S @ABCHDEDF||cK$.7< AH$E1BC5W*kc@ `S"UH  c 8@ABCDEFyd||&&xlR08AVfvhF3# .;HCVi+~Opn]H3 @ `S"|   S &@ABClDEF||##  2 p Fk,5>BJRdhl}ikeYaEU&ME52/_=H>1?0B$;4 $ @ `S"%F   S >@ABCDEF||))* !L'r6Edu,Mn}bz>hT@ {*@ `S"    S :@ABCDEF||((& *7BZ#r1HYj~ ?ewrM(wNV0D2 2&@ `S"F h   S @ABCDE|F|| -5BRWo %@GUcsyyf0~iFq3M <)  @ `S"Wb :P   S @ABCDEdFd|| AEia};en5o6{"J @ `S" z  c $@ABCdDEFd||""E<'41 *# 1R u+0 5h/0106<P}Wn^]b>cdc]WcDE<@ `S" DA 6  S @ABCHDELF||C;@Thp{ /{7q?nDZFFH#EC@ S"] w J  c @ABC[DEXFyd||E? =3))Rd v|'PA=K*U[Z YKE@ `S" f  c l@ABCDEFd||44VcW~=#zn^:PUB1&0 `@w15?97,I/[2~>GPaeiHhb\Hz>?4&&u&3;CIV@ `S" :f  S @ABtCbDE|F||/<$g J_t tttt(b,P0'123159bFHM.Tb_\J8@ S"fj  c @AB>CDEtFy|| '{zZG4  '25 8>"// <JYhlQJ9(@ `S""4`h  c @AB|CDEtFd||Vs]G-*L_r|zx#^.P3B838#<@AKUhxf@ S"U #b  c @AB CEDEpFd||T A (  #+(3Q8z=ED9C<2(  t6] g T @ `S"R]J  # @ABCxDEF"".Cq&;EOT Z`dinxxxtldT^H-<-'l!H+2'# @ `"Qj2^  # @AB CDEF&& 3?dK+STUOFE;b.   - <8K\S`mQTydZP PE:' @ `"h8  c ,@ABCsDEFd||## k8*3<ABCL;397468?EKX ]bc6fVisok3TPKmBmAw6+@ `S"XcX  C @ABC>5  C T@ABCHDEF||11gv  q ?w  (Qz<BH5`3-13363/0N1<f<<L50+q#*'$!^ @ `S"  C @AB_ C<DEF||AAQ <  $ (@ -2H<<<M40,W$$$!,-5.*1'$d&'7($ !L~6 zUZ(w4 x Y  C O  $ _ [ Q @ `S"  C H@ABC>DEF||,,e')**-0:<5>^=95I-$#!(`  0  + z>'d !$%e' @  `S"  B    LUSQdkYkHrh7h_    <   8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~    B S  \*  B    ^* ! B0#   ^*r  ZA@޽h ?Sumtextb TTrffl0___PPT10.},___PPT9RpRR Sumi PaintingZ+ 0 ** !!B*(  X   C @ABCs  ! # @ABCE@F||Y   #@`S"     @ABCDEdF&GshV #.9@Rdv~\B$ @ `"b_  S ,@ABCDEF 2||"" 'nHDi7nfK5$<Da-OBWb~f 3uYVF3 @  S"8b  S @ABCDEtF|| 1KgeM, "+.=Lgl|WG6@ S" ~4  S @ABCHDEDF||cK$.7< AH$E1BC5W*kc@ `S"UH  c 8@ABCDEFyd||&&xlR08AVfvhF3# .;HCVi+~Opn]H3 @ `S"|  S &@ABClDEF||##  2 p Fk,5>BJRdhl}ikeYaEU&ME52/_=H>1?0B$;4 $ @ `S"%F  S >@ABCDEF||))* !L'r6Edu,Mn}bz>hT@ {*@ `S"   S :@ABCDEF||((& *7BZ#r1HYj~ ?ewrM(wNV0D2 2&@ `S"F h  S @ABCDE|F|| -5BRWo %@GUcsyyf0~iFq3M <)  @ `S"Wb :P  S @ABCDEdFd|| AEia};en5o6{"J @ `S" z  c $@ABCdDEFd||""E<'41 *# 1R u+0 5h/0106<P}Wn^]b>cdc]WcDE<@ `S" DA 6  S @ABCHDELF||C;@Thp{ /{7q?nDZFFH#EC@ S"] w J  c @ABC[DEXFyd||E? =3))Rd v|'PA=K*U[Z YKE@ `S" f  c l@ABCDEFd||44VcW~=#zn^:PUB1&0 `@w15?97,I/[2~>GPaeiHhb\Hz>?4&&u&3;CIV@ `S" :f  S @ABtCbDE|F||/<$g J_t tttt(b,P0'123159bFHM.Tb_\J8@ S"fj  c @AB>CDEtFy|| '{zZG4  '25 8>"// <JYhlQJ9(@ `S""4`h  c @AB|CDEtFd||Vs]G-*L_r|zx#^.P3B838#<@AKUhxf@ S"U #b  c @AB CEDEpFd||T A (  #+(3Q8z=ED9C<2(  t6] g T @ `S"R]J  # @ABCxDEF"".Cq&;EOT Z`dinxxxtldT^H-<-'l!H+2'# @ `"Qj2^   # @AB CDEF&& 3?dK+STUOFE;b.   - <8K\S`mQTydZP PE:' @ `"h8   c ,@ABCsDEFd||## k8*3<ABCL;397468?EKX ]bc6fVisok3TPKmBmAw6+@ `S"XcX  C @ABC>)    C T@ABCHDEF||11gv  q ?w  (Qz<BH5`3-13363/0N1<f<<L50+q#*'$!^ @ `S"   C @AB_ C<DEF||AAQ <  $ (@ -2H<<<M40,W$$$!,-5.*1'$d&'7($ !L~6 zUZ(w4 x Y  C O  $ _ [ Q @ `S"  C H@ABC>DEF||,,e')**-0:<5>^=95I-$#!(`  0  + z>'d !$%e' @  `S"  B   LUSQdkYkHrh7h_    B - "   NUSQdkYkHroRh7h_   Bȴ `  \*  B    ^*  BP    ^*`  HA@ ?Sumtextb TTrff80___PPT10.}0 ~v0 x(  x x 0p     P*   x 0    \* d x c $?Dx  x 0   8USQdkYkHre,g7h_ ,{N~ ,{ N~ ,{V~ ,{N~   x 6     Z*  x 6    \* H  x 0޽h ? ̙3380___PPT10.P%~ @(    0/     P*    03    \*   6/     Z*   6    \* H  0޽h ? 3380___PPT10.>0N0 ZRP(    S 8p    xHQN7hTv'`( N,'`(Ջ7hvSOy0vˆ}v( TBg(vϑ0Ջ7hvpHSyP[:_^ vvirSyr'`'Y\0pI0S(solubility)0N4l'`0Sf['`( vvuSyr'`3z['`0RVP[ Hd '$ӜC'$kkY' kkF%   6d f,{mQ v~Sekv 2&$kk H  0޽h ? 3380___PPT10.}0N0 ph` (     S p<   Qv['` A._Y6k 7hTSOy0peϑ'Y HQQؚ[ϑcapacity vQ!kؚRsresolution Ytϑ\eRSKN B.vgTvSm^ؚ q_TyP[Nbcr1T5ul HQۏLgbQǏn1v0 Hj' ӜCa' kk' kkZ  %  s *Rl h1.Ryelvz^ 2 &$ӜCH  0޽h ? 3380___PPT10.}0N0 RJp(    s *;c  PlC Ryvk*Nek b N T'`(vRyel D gTRyagNl͑ YS'`0_ V:Nˆ}v(~3z['` NM_07 2 ' kk' ?' kk' ?' kk' ?' kk(H  0޽h ? 3380___PPT10.}\0N0 D(     S @,@   2.Rek~S N,lap A. )n^ B.MQgzI,pH C. g:gnBR D.ˆ}v(vSm^ؚ 3z[ V:Nˆ}v(&^5u ceR'Y E.MQllTvb_b F.vv;m'` HX' ӜC' kkJ' kk  H  0޽h ? 3380___PPT10.}&0N0 NF(     S 1v D<$D 0   z 6.~Sekv[ϑċN  '$ӜC 8  6TT ,$D 0 $3. b'`v~Sek r1lB 2&$ӜC&$8*&$ӜCf  6T,$D 0 P4.hg~SvۏU\`Q 8^(u5ulPAGE, SDS-PAGE, IEF 0) 2&$ӜC&$8*(  s *V$ ,$D 0 X5.ۏNek~S 9hnc4~g RP['Y\]_  GEC 5uw]_  yP[Nbcr10- 2 &$ӜC!&$8*P  R  C *A@?BD14868_F H  0޽h ? 33h`___PPT10@..}+[0D(' B=*@B BTimeNode0D' =*BTimeNode1@BA?%,( < +O%,( < +D)' =BTimeNode2%(D' =BTimeNode3%(D?' =^BTimeNode4BBB@B0B%(D' =BTimeNode51:Bvisible*o3>+B#style.visibility<*%(D6' =BTimeNode6+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*D.' =BTimeNode7+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*D5' =BTimeNode12%(D' =BTimeNode13%(DG' =`BTimeNode14BBB@B0B%(D' =BTimeNode151:Bvisible*o3>+B#style.visibility<*%(D8' =BTimeNode16+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*D0' =BTimeNode17+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*D5' =BTimeNode22%(D' =BTimeNode23%(DG' =`BTimeNode24BBB@B0B%(D' =BTimeNode251:Bvisible*o3>+B#style.visibility<*%(D8' =BTimeNode26+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*D0' =BTimeNode27+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*D5' =BTimeNode32%(D' =BTimeNode33%(DG' =`BTimeNode34BBB@B0B%(D' =BTimeNode351:Bvisible*o3>+B#style.visibility<*%(D8' =BTimeNode36+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*D0' =BTimeNode37+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*++0+0 ++0+0 ++0+0 ++0+0 + 0N0 (  r  S <    H  0޽h ? 3380___PPT10.}g 0N0 #% O(   r  S -     s *x1V 8The following information is known about four proteins: &97" 9 Rt   %  #"&    c $0   t4 identical, 50,000 each  @`   c $`F@   _5.8  @`   c $P @  c200,000  @`   c $0[   ]D  @`   c $c   ]1  @`   c $l@   _6.2  @`   c $v @  b35,500  @`   c $8   ]C  @`   c $  ]1  @`   c $@   _9.1  @`   c $`@  b14,200  @`   c $  ]B  @`   c $ఌ ]1  @`   c $@  _5.2  @`   c $8Č@  b13,100  @`   c $͌ ]A  @`   c $׌ dSubunits    @`   c $d@  ^pI  @`   c $@  ^Mr  @`   c $ cProtein  @`TB   c $oNB   S 1NB   S 1NB   S 1  NB   S 1  TB   c $o  TB   c $o NB   S 1 NB !  S 1@ @ NB "  S 1 TB #  c $o H  0޽h ? 3380___PPT10.} 0N0 $(  $r $ S   H $ 0޽h ? 3380___PPT10.} 0N0 ((  (r ( S   H ( 0޽h ? 3380___PPT10.}) 0N0 ,N(  , , S 0<$D 0    , S <$ 0   , NPAL$D 0c0H , 0޽h ? 33&&___PPT10z&..}+4D%' $=*@B BTimeNode0Dp%' =*BTimeNode1@BA?%,( < +O%,( < +D)' =BTimeNode2%(D' =BTimeNode3%(D?' =^BTimeNode4BBB@B0B%(D' =BTimeNode51:Bvisible*o3>+B#style.visibility<*,%(D6' =BTimeNode6+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*,D.' =BTimeNode7+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*,D(' =BTimeNode12%(D' =BTimeNode13%(D:' =SBTimeNode14BBB@B%(D' =BTimeNode151:Bvisible*o3>+B#style.visibility<*,%(D8' =BTimeNode16+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*,D0' =BTimeNode17+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*,D5' =BTimeNode18%(D' =BTimeNode19%(DG' =`BTimeNode20BBB@B0B%(D' =BTimeNode211:Bvisible*o3>+B#style.visibility<*,%(D8' =BTimeNode22+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*,D0' =BTimeNode23+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*,D5' =BTimeNode24%(D' =BTimeNode25%(DG' =`BTimeNode26BBB@B0B%(D' =BTimeNode271:Bvisible*o3>+B#style.visibility<*, %(D8' =BTimeNode28+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*, D0' =BTimeNode29+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*, D5' =BTimeNode30%(D' =BTimeNode31%(DG' =`BTimeNode32BBB@B0B%(D' =BTimeNode331:Bvisible*o3>+B#style.visibility<*, %(D8' =BTimeNode34+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*, D0' =BTimeNode35+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*, D5' =BTimeNode36%(D' =BTimeNode37%(DG' =`BTimeNode38BBB@B0B%(D' =BTimeNode391:Bvisible*o3>+B#style.visibility<*,"%(D8' =BTimeNode40+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*,"D0' =BTimeNode41+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*,"D5' =BTimeNode42%(D' =BTimeNode43%(DG' =`BTimeNode44BBB@B0B%(D' =BTimeNode451:Bvisible*o3>+B#style.visibility<*,")%(D8' =BTimeNode46+4 8?dCB0-#ppt_w/2BCB#ppt_xB*Y3>B ppt_x<*,")D0' =BTimeNode47+4 8?\CB#ppt_yBCB#ppt_yB*Y3>B ppt_y<*,")+p+0+,0 ++0+,0 +$ 0 0$(  0r 0 S 5:   r 0 S 8   H 0 0޽h ? 3380___PPT10.}8 0 48(  4r 4 S 0=    4 S @H    H 4 0޽h ? 3380___PPT10.}8 0 88(  8r 8 S LF     8 S b    H 8 0޽h ? 3380___PPT10.}< 0N0  <$(  <r < S $`   r < S z   H < 0޽h ? 3380___PPT10.}$ 0 0$(  r  S    r  S `QTt   H  0޽h ? 3380___PPT10.uo< 0N0 @@$(  @r @ S 3   r @ S C  H @ 0޽h ? 3380___PPT10.}0N0 rjPD(  D^ D c 6A@ 3?gv\O(uv!j_7.B D  ?p@.B D  ? 0 H D 0޽h ? 3380___PPT10.} 0N0 `H(  H H  Bvd @   2 H 0d'% Nˆ}v((2"(  H c $X-`@  Jv(2"( r H s *Ԑ?` L N(2&(-  H S :<$ 0  H H 0޽h ? 33___PPT10..}+TD#' =*@B BTimeNode0D' =*BTimeNode1@BA?%,( < +O%,( < +D' =BTimeNode2%(D' =BTimeNode3%(D' =^BTimeNode4BBB@B0B%(D' =BTimeNode51:Bvisible*o3>+B#style.visibility<*H%(D' =BTimeNode6-s6Bwipe(left)*<3<*HD' =BTimeNode7%(D' =BTimeNode8%(D' =^BTimeNode9BBB@B0B%(D' =BTimeNode101:Bvisible*o3>+B#style.visibility<*H/%(D' =BTimeNode11-s6Bwipe(left)*<3<*H/D' =BTimeNode12%(D' =BTimeNode13%(D' =`BTimeNode14BBB@B0B%(D' =BTimeNode151:Bvisible*o3>+B#style.visibility<*H/7%(D' =BTimeNode16-s6Bwipe(left)*<3<*H/7D' =BTimeNode17%(D' =BTimeNode18%(D' =`BTimeNode19BBB@B0B%(D' =BTimeNode201:Bvisible*o3>+B#style.visibility<*H7?%(D' =BTimeNode21-s6Bwipe(left)*<3<*H7?D' =BTimeNode22%(D' =BTimeNode23%(D' =`BTimeNode24BBB@B0B%(D' =BTimeNode251:Bvisible*o3>+B#style.visibility<*H?T%(D' =BTimeNode26-s6Bwipe(left)*<3<*H?T+8+0+H0 +z 0N0 pLb(  L L <! ` PH@___PPT9" RVh (QB547-80) Stˆ}vv4ljˆ}v vQNir-N+TZWv(lWx(Wx'`agN Nyg (Folin) ՋBR؏S ub݄rSTir N݄rvmEmKmwv;mRv'Y\0>I ZG &(?G& 8*2 "R L C *A@?BD00028_X:B L@ 3 bԔ`4B L # bԔ`0`4B L # bԔ0H L 0޽h ? 3380___PPT10.}z 0N0 Pb(  PP P  lE0e0eDEX ? ?`T`T`T`T`0p<$ 0   P c $ ~"-N'`ˆ}vvAS1398 ;mRvKm[(2"(H P 0޽h ? 3380___PPT10.} 0N0   T(  Tr T S    4B T # PP  T s *  L;`SOy 2! 4B T@ #  p@0  T s * 0 P  NS^e 2! 4B T #   `  T c $   *hQf~-NO.D+B#style.visibility<*d %(D' =BTimeNode6-s6Bwipe(left)*<3<*d D' =BTimeNode7%(D' =BTimeNode8%(D' =^BTimeNode9BBB@B0B%(D' =BTimeNode101:Bvisible*o3>+B#style.visibility<*d %(D' =BTimeNode11-s6Bwipe(left)*<3<*d +8+0+d0 +< 0N0 h$(  hr h S `   r h S LR  H h 0޽h ? 3380___PPT10.}$ 0 l$(  lr l S 8X   r l S Z   H l 0޽h ? 3380___PPT10.}5- 0N0 ,,DOp,(  p*  Op #"2&@?iihihi p c $lo" T  @` p c $p 3 1] ] BB ?p 3 13 3 BB @p 3 1BB Ap 3 1HB Bp C o Cp 62vR > 2!  Dp s *p XǏ'lSirv~Sh 2 #$ H p 0޽h ? 3380___PPT10.}$. 0N0 |-t-DD -(  n+   #"2&@?iihihi  c $|" a628.4  @`  c $4" b4272.7  @`  c $Ȥ3 " `0.33  @`  c $pФ] "3  ]4  @`  c $٤"]  `1410  @`  c $" DEAE-Sephadex g @`   c $ " a199.0  @`   c $ " b1352.9  @`   c $x3  " _1.7  @`   c $]  3 " ]7  @`   c $ ] " `2300  @`  c $  " .Phenyl-Sepharose CL-4Bg @`  c $   a115.0  @`  c $$   a782.0  @`  c $|,3   _5.9  @`  c $4] 3   ^14  @`  c $`= ]   `4614  @`  c $E    ConA-Sepharose g @`  c $DNL   _8.2  @`  c $|WL   `56.0  @`  c $_3 L   _308  @`  c $`h] L 3  ^51  @`  c $pL ]  a17253  @`  c $|yL   DEAE-Sephadex g @`  c $dL  _2.5  @`  c $L  `17.3  @`  c $|3 L  `1474  @`  c $] 3 L  ^76  @`  c $d] L  a25560  @`   c $ԫL  Bio-Gel P-60g  @` ! c $ _1.4  @` " c $D _9.8  @` # c $ģ3  `3300  @` $ c $lͣ] 3  ^96  @` % c $գ]  a32200  @` & c $Pޣ 10%kxxqT^ Nnm  @` ' c $ _1.0  @` ( c $p _6.8  @` ) c $3  `4982  @` * c $P] 3  _100  @` + c $ ]  a33652  @` , c $ d|mS  @` - c $ h~S Ppe?  @` . c $# k;mRvUSMO/mgˆ}v( (?  @` / c $D,3  x;`vˆ}v((mg)   @` 0 c $5] 3  NϑbV6es%   ? @` 1 c $@]  n;`vv;mRUSMO     @` 2 c $I dRyel  @`HB 3 C oBB 4 3 1BB 5 3 1BB 6 3 1BB 7 3 1L L BB 8 3 1  BB 9 3 1  BB : 3 1""HB ; C oHB < C oBB = 3 1BB > 3 1] ] BB ? 3 13 3 BB @ 3 1BB A 3 1HB B C o C 6Q2vR > 2!  D s *4Wp XǏ'lSirv~Sh 2 #$ H  0޽h ? 3380___PPT10.}ٍ0N0 -- ;M-(    0f1"  $,$D 0  6df1" c,$D 0  6df1"  ,$D 0  s *f1/,$D 0zB  S <<,$D 0F  He1pq,  % |Tbvv~ShP g  fc  C c  &  hh88xl  0P,$D 0   0{ff1 ~Sek< %  0  hh88   0ff1#`   hQˆ}v( D'  ' &  hh88   0ff1 vV6es '  hh88   0ff1   x hQ;m'` '  hh88   0pff1 1 vk;m'` '  hh88  0ff1]  ~S Ppe 0 '  hh88Z  H趧1q,$  0 |bcm>" "  " &  hh88~  H辧10q ,$  0 ||ˆ}vlmT Nn J "  " " 0  hh88  HLا1q ,$  0  kxxR~ (35-55%) >"  "  " 4    hh88n  H1 q ,$  0 *Sepharose CL-6B QǏn0" " &  hh88n  H,1 q,$  0 *DEAE Sepharose yP[Nbc 0" " &  hh88l "K  `  ,$D 0BB  3 fg K K BB  3 fg ""BB  3 fg k k BB  3 fg   BB  3 fg {l   ]j,$D 0  H1  y(mg) $" "  hh88  H1 &  x(U) $" "  hh88  H#1w   {(U/mg) $" "  hh88  Hx/1 =  {(fold) $" "  hh88  H`51H  x(%) $" "  hh88}l F   G ),$D 0 ! HE1 z1,070 $" "  hh88 " HdP1G=J  z800 &" "  hh88 # H\1G *  z250 &" "  hh88 $ HLX1   y53 &" "  hh88 % Hlm1 F  z8.6 &" "  hh88l :   &0 ),$@ 0 ' H|~1   z9,672 $" "  hh88 ( H1: = J  12,555 ,& &  hh88 ) H1  *  z6,610 $" "  hh88 * Hl1  z5,789 $" "  hh88 + H1  z2,960 $" "  hh88 > H 1,$ 0 F bc 100 g 4l;zbqg7| &  & & & d     hh88l + ?,$D 0BB @ 3 o++BB A 3 onB B 3 ,$D 0nB C 3 8 ,$D 0tB D C 8 8 ,$D 0hB E # h h ,$D 0hB F # h ,$D 0nB G 3 ,$D 0nB H 3 jJ,$D 0nB I 3 jJ,$D 0tB J C jJ,$D 0tB K C 1 ,$D 0tB L C 1 ,$D 0zB M S 1,$D  0H  0޽h ? 33n_f____PPT10F_.}+qHD]' =*@B BTimeNode0Dh]' =*BTimeNode1@BA?%,( < +O%,( < +D&' =BTimeNode2%(%(D' =BTimeNode3%(D' =QBTimeNode4BBB@B%(D' =BTimeNode51:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode6-u6Bwipe(right)*<3<*D' =BTimeNode7%(D' =QBTimeNode8BBB@B%(D' =BTimeNode91:Bvisible*o3>+B#style.visibility<*?%(D' =BTimeNode10-s6Bwipe(left)*<3<*?D ' =BTimeNode11%(D' =SBTimeNode12BBB@B%(D' =BTimeNode131:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode14-u6Bwipe(right)*<3<*D' =BTimeNode15%(D' =SBTimeNode16B BB@B%(D' =BTimeNode171:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode18-{6Bslide(fromTop)*<3<*D' =BTimeNode22%( D' =SBTimeNode23B BB@B%(D' =BTimeNode241:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode25-6B%slide(fromBottom)*<3<*D$' =BTimeNode29%( D' =`BTimeNode30B BB@B0B%(D' =BTimeNode311:Bvisible*o3>+B#style.visibility<*>%(D' =BTimeNode32-6B#slide(fromRight)*<3<*>D' =BTimeNode36%(D' =SBTimeNode37BBB@B%(D' =BTimeNode381:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode39-o6Bwipe(up)*<3<*D"' =BTimeNode40%(D' =`BTimeNode41B BB@B0B%(D' =BTimeNode421:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode43-}6B!slide(fromLeft)*<3<*D"' =BTimeNode47%(pD' =`BTimeNode48B BB@B0B%(D' =BTimeNode491:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode50-}6B!slide(fromLeft)*<3<*D"' =BTimeNode54%(XD' =`BTimeNode55B BB@B0B%(D' =BTimeNode561:Bvisible*o3>+B#style.visibility<*%(D' =BTimeNode57-}6B!slide(fromLeft)*<3<*D"' =BTimeNode61%(@D' =`BTimeNode62B BB@B0B%(D' 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Nn J "  " " 0  hh88 t H1q ,$  0  kxxR~ (35-55%) >"  "  " 4    hh88n t H1 q ,$  0 *Sepharose CL-6B QǏn0" " &  hh88n t Hħ1 q,$  0 *DEAE Sepharose yP[Nbc 0" " &  hh88l "K  t`  ,$D 0BB t 3 fg K K BB t 3 fg ""BB t 3 fg k k BB t 3 fg   BB t 3 fg {l   t]j,$D 0 t H1  y(mg) $" "  hh88 t H˯1 &  x(U) $" "  hh88 t Hӯ1w   {(U/mg) $" "  hh88 t HPݯ1 =  {(fold) $" "  hh88 t H@1H  x(%) $" "  hh88}l F   tG ),$D 0 !t H|1 z1,070 $" "  hh88 "t H 1G=J  z800 &" "  hh88 #t H1G *  z250 &" "  hh88 $t H1   y53 &" "  hh88 %t H01 F  z8.6 &" "  hh88l :   &t0 ),$@ 0 't HL+1   z9,672 $" "  hh88 (t H91: = J  12,555 ,& &  hh88 )t HD1  *  z6,610 $" "  hh88 *t HxL1  z5,789 $" "  hh88 +t HT1  z2,960 $" "  hh88zl   ,t ),$@ 0 -t He1Q x9.0 $" "  hh88 .t HP1 =J  y15.7 $" "  hh88 /t HD{1  *  y26.4 $" "  hh88 0t H1   z111.3 $" "  hh88 1t H81  z344.2 $" "  hh88vl 7  2tF),$@ 0 3t H1  x1.0 $" "  hh88 4t H\1= J  x1.7 $" "  hh88 5t Hx1 *  x2.9 $" "  hh88 6t H17   y12.4 $" "  hh88 7t H17  y38.2 $" "  hh88l 2) 8t2),$D! 0 9t H12 y100&" 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=UBTimeNode116BBB@B%(D' =!BTimeNode1171:Bvisible*o3>+B#style.visibility<*t%(D' =!BTimeNode118-o6Bwipe(up)*<3<*tDX' =!BTimeNode119%(D' =!BTimeNode120%(D' =UBTimeNode121BBB@B%(D' =!BTimeNode1221:Bvisible*o3>+B#style.visibility<*Bt%(D' =!BTimeNode123-o6Bwipe(up)*<3<*BtD' =!BTimeNode124%(D' =UBTimeNode125BBB@B%(D' =!BTimeNode1261:Bvisible*o3>+B#style.visibility<*Ct%(D' =!BTimeNode127-s6Bwipe(left)*<3<*CtD' =!BTimeNode128%(D' =UBTimeNode129BBB@B%(D' =!BTimeNode1301:Bvisible*o3>+B#style.visibility<*Dt%(D' =!BTimeNode131-s6Bwipe(down)*<3<*DtD' =!BTimeNode132%(D' =UBTimeNode133BBB@B%(D' =!BTimeNode1341:Bvisible*o3>+B#style.visibility<*Et%(D' =!BTimeNode135-o6Bwipe(up)*<3<*EtD' =!BTimeNode136%( D' =UBTimeNode137BBB@B%(D' =!BTimeNode1381:Bvisible*o3>+B#style.visibility<*Ft%(D' =!BTimeNode139-s6Bwipe(left)*<3<*FtD' =!BTimeNode140%( D' =UBTimeNode141BBB@B%(D' =!BTimeNode1421:Bvisible*o3>+B#style.visibility<*Gt%(D' =!BTimeNode143-s6Bwipe(down)*<3<*GtD' =!BTimeNode144%( D' =UBTimeNode145BBB@B%(D' =!BTimeNode1461:Bvisible*o3>+B#style.visibility<*Ht%(D' =!BTimeNode147-o6Bwipe(up)*<3<*HtD' =!BTimeNode148%(D' =UBTimeNode149BBB@B%(D' =!BTimeNode1501:Bvisible*o3>+B#style.visibility<*It%(D' =!BTimeNode151-s6Bwipe(left)*<3<*ItD' =!BTimeNode152%(D' =UBTimeNode153BBB@B%(D' =!BTimeNode1541:Bvisible*o3>+B#style.visibility<*Jt%(D' =!BTimeNode155-s6Bwipe(down)*<3<*JtD ' =!BTimeNode156%(D' =!BTimeNode157%(D' =UBTimeNode158BBB@B%(D' =!BTimeNode1591:Bvisible*o3>+B#style.visibility<*Kt%(D' =!BTimeNode160-o6Bwipe(up)*<3<*KtD' =!BTimeNode161%(D' =UBTimeNode162BBB@B%(D' =!BTimeNode1631:Bvisible*o3>+B#style.visibility<*Lt%(D' =!BTimeNode164-s6Bwipe(left)*<3<*LtD' =!BTimeNode165%(D' =UBTimeNode166BBB@B%(D' =!BTimeNode1671:Bvisible*o3>+B#style.visibility<*Mt%(D' =!BTimeNode168-s6Bwipe(down)*<3<*MtD' =!BTimeNode169%(D' =UBTimeNode170B BB@B%(D' =!BTimeNode1711:Bvisible*o3>+B#style.visibility<*8t%(D' =!BTimeNode172-{6Bslide(fromTop)*<3<*8t+P+0+t0 ++0+t0 ++0+t0 ++0+t0 ++0+t0 ++0+>t0 +{ 0N0 @c(  r  S T- `     s *|q@  W Problem set 1(2" H  0޽h ? 3380___PPT10.}0 P&(  R  3 eK    C 0gq   4 feW[  H  0޽h ? ̙3380___PPT10.~0 `|&(  |R | 3 eK   | C Lgq   4 feW[  H | 0޽h ? ̙3380___PPT10.~rxp^RЀ3ÿ lHbP  @AL G@;b bW< ]P^b (y,2#T@C~Xr5<UlCMmoӰ:R"($K<@ODJWL!NeP[;^g`лIv# (1Oh+'0t hp(<    ˽Ʒ һʣøʣʵpHǿ øﻯԣСpIS(solubility)ˮԡѧ øԣȶԡ  BillgatesTC:\Program Files\Microsoft Office\Templates\Presentation Designs\Sumi Painting.pottianer8Microsoft Office PowerPoint@χ@E/@ +Ghg  ,V'&" WMFC <8?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_abcdefgijklmnoxRoot EntrydO)PicturesCurrent UserhSummaryInformation(PowerPoint Document(DocumentSummaryInformation8`