寡肽-5和KGF-2是什么？与FGF-10有什么联系？

youngshe2015

寡肽-5和KGF-2是什么？与FGF-10有什么联系？

重组人角质细胞生长因子（rh-KGF）就是FGF-10的全称，英语的全称为：sh-polypeptide-10，而寡肽-5则是在中国的简称，有时也叫人寡肽-5




品 名：重组人角质细胞生长因子（rh-KGF）

分 子 量：19.2kD

规 格：10μg、100μg、1mg

形式：冻干粉

保存条件：-20℃保存、3年

介绍： 重组人角质细胞生长因子（rh-KGF）由原核表达系统（E.coli）重组表达制备，由169个氨基酸残基组成的非糖基化多肽，分子量为19.2kDa。重组人角质细胞生长因子（rh-KGF）从属于成纤维细胞生长因子家族。rh-KGF基因表达受多种细胞因子调控。rh-KGF与受体KGFR特异性的结合发挥其多种生物学功能：参与组织、器官的发育；参与组织、器官的发育；参与皮肤、胃、肠、肾、膀胱、肺等上皮的损伤修复；减少放、化疗所带来的副作用，具有损伤防护功能。
Keratinocyte growth factor (KGF), which is also called fibroblast growth factor (FGF)-7, belongs to the FGF family. KGF is not commonly produced by human cancer cells, but the KGF receptor (KGFR) is expressed in most cancer cells and particularly highly expressed in well-differentiated types of cancer.




Recently, it has been reported that vascular endothelial growth factor (VEGF)-A expression is induced by KGF in pancreatic cancer cells. VEGF-A is produced by some cancer cells and plays important roles in the angiogenesis and metastasis of cancer cells including those in the colorectum.

In this study, we examined whether recombinant human KGF (rhKGF) induces major angiogenic growth factors including VEGF-A, FGF-2 and hepatocyte growth factor (HGF) in human colorectal cancer cells (HCT-15), which express a high level of KGFR, but a low or negligible level of KGF. rhKGF significantly increased the VEGF-A expression level in a serum-free medium of HCT-15 cells, but FGF-2 and HGF expression levels were too low to detect.




Furthermore, the expression levels of the angiogenic growth factors were evaluated in KGF-transfected HCT-15 cells, which were induced to stably overexpress KGF by KGF gene transfection and mock-transfected cells (Mock).

KGF and VEGF-A expression levels in the cells and the protein concentrations in serum-free medium were significantly higher in KGF-transfected HCT-15 cells than in Mock cells. In contrast, the FGF-2 and HGF mRNA expression levels were not significantly different between KGF-transfected HCT-15 cells and Mock cells and the protein concentrations in serum-free medium of the cells were below the detection level.

These findings suggest that administration of rhKGF and over-expression of endogenous KGF genes in colorectal cancer cells increase VEGF-A production and may relate to angiogenesis in cancer.

使用说明：

1、KGF冻干粉稳定性一般，冻干粉溶解后活性可稳定7d内；

2、用于修复伤口或伤疤时，注意不要污染配置好的溶液；

3、根据需要修复的面积计算使用KGF的量，按照每平方分米（约等于人面部面积）使用2000-5000IU（2-5μg）；

4、使用时注意保持无菌不污染产品。