pigtype CSFV Erns Ab ELISA 猪瘟Erns抗体检测试剂盒

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pigtype erns ab ELISA 猪瘟csfv erns 抗体检测试剂盒

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For detection of antibodies to the Erns protein of the classical swine fever virus (CSFV)

The pigtype CSFV Erns Ab is a double-antigen ELISA. The microtiter plate is coated with recombinant CSFV Erns antigen. During sample incubation, antibodies to the CSFV Erns protein bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies to the CSFV Erns protein are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620-650 nm) is optional. The OD value correlates to the concentration of the antibodies to the CSFV Erns protein in the sample.